Recent developments for SPIE-IA, a new sandwich immunoassay format for very small molecules

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 34; no. 4; pp. 737 - 752
• Main Authors: Volland, Hervé, Pradelles, Philippe, Taran, Frédéric, Buscarlet, Laure, Creminon, Christophe
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01-03-2004
• Subjects: Antioxidants - analysis; Antioxidants - pharmacology; Cell culture; Cross-Linking Reagents - analysis; Epitopes - analysis; Epitopes - immunology; France; Free radicals; Free Radicals - analysis; Immune complex; Immunoassay - methods; Immunoassay - trends; Irradiation; Models, Immunological; Noncompetitive assays; Oxidant stress; Small analytes; Ultraviolet Rays - adverse effects; France; Cell culture; Free radicals; Noncompetitive assays; Irradiation; Small analytes; Oxidant stress; Immune complex
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/S0731-7085(03)00560-0

Recent publications describing new elegant approaches to assay small analytes using noncompetitive format were briefly reviewed. Among these methods, we have developed a new protocol, named SPIE-IA, which involves a cross-linking step achieved using chemical hombifunctional reagents, UV irradiation or free radicals. This new method proved to be useful to detect naturally occurring analyte/antibody complexes or to protect the analytes against degradation by peptidases. On the other hand, SPIE-IA could allow to study the adverse biological effects of UV and some aspects of free radical chemistry or to evaluate the antioxidant activity of molecules.