Identification of Ruscus steroidal saponins by HPLC-MS analysis

• Published in: Fitoterapia Vol. 73; no. 7; pp. 583 - 596
• Main Authors: de Combarieu, E., Falzoni, M., Fuzzati, N., Gattesco, F., Giori, A., Lovati, M., Pace, R.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01-12-2002; Elsevier
• Subjects: 1- O-[α- l-rhamnopyranosyl-(1→2)-6- O-acetyl-β- d-galactopyranosyl]-1β,3β,22ξ,26-tetrahydroxy-furost-5- en-26- O-β- d-glucopyranoside; Biological and medical sciences; Chromatography, High Pressure Liquid; General pharmacology; HPLC-ESI-MS; HPLC-UV; Mass Spectrometry; Medical sciences; Molecular Structure; Neoruscogenin; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plant Leaves - chemistry; Rhizome - chemistry; Ruscin; Ruscogenin; Ruscoside; Ruscus - chemistry; Ruscus - classification; Ruscus aculeatus; Ruscus colchicus; Ruscus hypoglossum; Saponins - analysis; Saponins - chemistry; Asia; Turkey; Ruscoside; Ruscus aculeatus; Ruscus colchicus; 1- O-[α- l-rhamnopyranosyl-(1→2)-6- O-acetyl-β- d-galactopyranosyl]-1β,3β,22ξ,26-tetrahydroxy-furost-5- en-26- O-β- d-glucopyranoside; Ruscogenin; HPLC-ESI-MS; Neoruscogenin; Ruscin; HPLC-UV; Ruscus hypoglossum; Monocotyledones; HPLC chromatography; Medicinal plant; Saponin; Angiospermae; Rhizome; 1-O-[α-L-rhamnopyranosyl-(1→2)-6-O-acetyl-β-D-galactopyranosyl]-1β,3β,22ξ,26-tetrahydroxy-furost-5-en-26-O-β-D-glucopyranoside; Qualitative analysis; Pharmacognosy; Antioxidant; In vitro; Biological activity; Liliaceae; Ultraviolet detector; Fingerprint method; Plant origin; Isolation; Active ingredient; Spermatophyta; Structural analysis
• ISSN: 0367-326X
• DOI: 10.1016/S0367-326X(02)00220-4

A novel HPLC-UV method has been developed for the fingerprint analysis of the steroidal saponins in the rhizomes of three
Ruscus species (
Ruscus aculeatus,
Ruscus hypoglossum and
Ruscus colchicus). Saponins were identified by HPLC-ESI-MS. During the study a new major saponin was detected in the rhizomes of
R. hypoglossum and
R. colchicus. The structure of the new compound was defined as 1-
O-[α-
l-rhamnopyranosyl-(1→2)-6-
O-acetyl-β-
d-galactopyranosyl]-1β,3β,22ξ,26-tetrahydroxy-furost-5(6)-
en-26-
O-β-
d-glucopyranoside (
8) by spectral analysis.