Genetic Diversity and Pedigree Analysis of Red Currant Germplasm

• Published in: Plants (Basel) Vol. 11; no. 13; p. 1623
• Main Authors: Pikunova, Anna, Goryunova, Svetlana, Goryunov, Denis, Golyaeva, Olga, Dolzhikova, Maria, Pavlenko, Anna
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 21-06-2022; MDPI
• Subjects: breeding; Classification; Clustering; Cultivars; Desserts; Genetic analysis; genetic collection; Genetic diversity; Genomes; Genotypes; Genotyping; Germplasm; Multidimensional scaling; Offspring; Pedigree; Plant reproduction; Polymorphism; red currant; Ribes petraeum; Ribes rubrum L; varieties; France; Europe; Germany
• DOI: 10.3390/plants11131623
• EISSN: 2223-7747

This represents the first report on the genetic diversity of red currant germplasm collections based on genotyping-by-sequencing (GBS) data. Genotypes of 75 individuals of different origin were assessed in more than 7.5K genome positions. Multidimensional scaling (MDS) analysis has been performed. There are five accessions that are significantly isolated from each other and from the rest of the analyzed cultivars. F1 offspring of R. petraeum Wulf (Rote Hollandische) and Gondouin, as well as Rote Spatlese (F2 of R. petraeum and F2 of R. multiflorum Kit.), are the most genetically isolated on the MDS plot. Ribes multiflorum is closer to the rest of cultivars than the three abovementioned accessions. Purpurnaya cultivar (F1 of Rote Spatlese) is located between Rote Hollandische and R. multiflorum. Other genotypes, mostly represented by varieties having several species in a pedigree, occupied the rest of MDS plot relatively evenly. Descendants of R. multiflorum have been placed in the left part of MDS plot, which underlines their genetic diversity from other accessions. White- and pink-fruited cultivars were clustered together, underlining genetic relatedness. Admixture analysis of GBS data reveals six clusters (K = 6). Presumably, clustering reflects relatedness to R. petraeum, R. rubrum, R. vulgare var macrocarpum, R. multiflorum, R. vulgare, and Jonker van Tets. Based on genotyping data, F1 offspring of R. warscewiczs Jancz (cultivar Viksne), R. altissimum Turcz (Cirald), and R. palczewskii (Jancz.) Pojark (Skorospelaya) have not exhibited strict separation and were placed in a pool with other varieties. This supports modern taxonomic classifications that do not consider R. altissimum and R. palczewskii as independent species.