Cloning, sequencing and structural analysis of 976 base pairs of the promoter sequence for the rat lipoprotein lipase gene. Comparison with the mouse and human sequences

• Published in: Gene Vol. 209; no. 1; pp. 31 - 38
• Main Authors: Bey, Lionel, Etienne, Jacqueline, Tse, Chantal, Brault, Didier, Noé, Lydie, Raisonnier, Alain, Arnault, Frédéric, Hamilton, Marc T, Galibert, Francis
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 16-03-1998
• Subjects: Animals; Base Composition; Base Sequence; Cloning, Molecular; DNA-Binding Proteins - metabolism; Genetic Variation; Humans; Lipoprotein Lipase - biosynthesis; Lipoprotein Lipase - genetics; Mice; Molecular Sequence Data; Potential cis-elements; Promoter Regions, Genetic; Rat insert; Rats - genetics; Regulatory Sequences, Nucleic Acid; Restriction Mapping; Restriction sites; Sequence Alignment; Sequence Homology, Nucleic Acid; Stem loops; Transcription, Genetic; Transition; Transversion; Rat insert; VLDL, very low-density lipoprotein; Transition; Potential cis-elements; Transversion; Stem loops; PCR, polymerase chain reaction; nt, nucleotide; LPL, lipoprotein lipase; Restriction sites
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/S0378-1119(98)00003-1

We cloned and sequenced the −976
bp promoter of the rat lipoprotein lipase
LPL gene. The sequence was compared with the mouse and human sequences. The homology between the rat and mouse
LPL nucleotide sequences was not quite as strong in the promoter sequence as in the coding sequence. Among the 976
nt promoter there were 118 divergences, i.e. 11.8%, compared to only 5.6% for the LPL coding region. However, within the 200
nt immediately 5″ to the transcriptional start site (proximal promoter), the divergence was only 4%. New potential cis-elements (such as CACCC, GATA, GC and GA boxes, IRS, Krox, MEF-2, E-box, CCArGG and 1/2 VDRE) were identified in the rat, mouse or human
LPL gene.