GnRH Molecular Variants in the Brain and Pituitary Gland of Pejerrey, Odontesthes bonariensis (Atheriniformes). Immunological and Chromatographic Evidence for the Presence of a Novel Molecular Variant

• Published in: Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology Vol. 118; no. 3; pp. 335 - 345
• Main Authors: Stefano, Andrea V., Canosa, Luis F., D'Eramo, José L., Fridman, Osvaldo, Affanni, Jorge M., Somoza, Gustavo M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-11-1997
• Subjects: Animals; Argentina; Brain - metabolism; Brain Chemistry; Chromatography, High Pressure Liquid; Fishes; GnRH; Gonadotropin-Releasing Hormone - analogs & derivatives; Gonadotropin-Releasing Hormone - analysis; Gonadotropin-Releasing Hormone - chemistry; Gonadotropin-Releasing Hormone - isolation & purification; Gonadotropin-Releasing Hormone - metabolism; hypophysis; neuroendocrinology; neuropeptides; Odontesthes bonariensis; Pituitary Gland - chemistry; Pituitary Gland - metabolism; Protein Precursors - analysis; Protein Precursors - chemistry; Protein Precursors - metabolism; Radioimmunoassay; reproduction; RP-HPLC; teleost fish; Tissue Extracts - chemistry; Argentina; RP-HPLC; neuroendocrinology; radioimmunoassay; neuropeptides; hypophysis; teleost fish; GnRH; reproduction
• ISSN: 0742-8413; 1367-8280
• DOI: 10.1016/S0742-8413(97)00135-7

Gonadotropin-releasing hormone (GnRH) molecular variants in the brain and pituitary gland of pejerrey,
Odontesthes bonariensis (Atheriniformes), were characterized by gradient reverse phase high performance liquid chromatography (RP-HPLC). Eluted fractions were tested in radioimmunoassays with different antisera. The results show that the brain extract contains three forms of GnRH: one is immunologically and chromatographically similar to cIIGnRH (chicken II), and another is similar to sGnRH (salmon). A third GnRH appears to be chromatographic and immunologically different from the nine other known forms of the vertebrate hormone. This is the only variant present in the pituitary gland.