Determination of phospholipid fatty acids in biological samples by solid-phase extraction and fast gas chromatography

• Published in: Journal of Chromatography A Vol. 1116; no. 1; pp. 204 - 208
• Main Authors: Bondia-Pons, I., Morera-Pons, S., Castellote, A.I., López-Sabater, M.C.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 26-05-2006; Elsevier
• Subjects: Analytical chemistry; Biological and medical sciences; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, Gas - methods; Erythrocytes - chemistry; Exact sciences and technology; Fast-GC; Fatty acids; Fatty Acids - analysis; Gas chromatographic methods; Humans; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Miscellaneous. Technology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Phospholipids; Phospholipids - chemistry; Reference Standards; Reproducibility of Results; SPE; SPE; Fast-GC; Phospholipids; Fatty acids; Trace analysis; Human; Validation; Solid phase extraction; Biological fluid; Chemical analysis; Red blood cell; Phospholipid; Complex lipid; Derivatization; Blood; Chemical enrichment; Blood plasma; Gas chromatography; Sample preparation; Clinical biology; Chemical composition; Fatty acid methyl ester
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2006.03.023

A new method for the determination of phospholipid fatty acids in biological samples, combination of solid-phase extraction (SPE) and fast gas chromatography (GC) was developed. Its application to human plasma and human erythrocytes showed to be robust and reliable for quick and correct identification in routine analysis.