Resolution of allelic and non-allelic variants of histone H1 by cation-exchange-hydrophilic-interaction chromatography

• Published in: Journal of chromatography. B, Biomedical sciences and applications Vol. 744; no. 1; pp. 33 - 46
• Main Authors: Mizzen, Craig A, Alpert, Andrew J, Lévesque, Lyne, Kruck, Theo.P.A, McLachlan, Donald R
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 07-07-2000
• Subjects: Acetonitriles - chemistry; Alleles; Amino Acid Sequence; Animals; Chickens; Chromatography, High Pressure Liquid - methods; Chromatography, Ion Exchange - methods; Erythrocytes - chemistry; Histone H1; Histones - analysis; Histones - genetics; Molecular Sequence Data; Polymorphism, Genetic; Quality Control; Sequence Homology, Amino Acid; Histone H1
• ISSN: 0378-4347; 1387-2273
• DOI: 10.1016/S0378-4347(00)00210-3

A mixed-mode high-performance liquid chromatography (HPLC) method that resolves the six known non-allelic variants of chicken erythrocyte histone H1 is described. Common, but previously unknown, allelic variants of H1 that comigrate in polyacrylamide gel electrophoresis are also resolved. The resolution of H1 variants achieved by this method should be useful in determining the functional significance of H1 sequence heterogeneity and in analyses of post-translational modification of H1. Furthermore, the principles behind the separation should be applicable to analyses of polymorphism in other proteins.