Accelerated antigen presentation and elicitation of humoral response in vivo by FcγRIIB- and FcγRI/III-mediated immune complex uptake

• Published in: Cellular immunology Vol. 225; no. 1; pp. 21 - 32
• Main Authors: Yada, Ayumi, Ebihara, Shin, Matsumura, Kimio, Endo, Shota, Maeda, Tsutomu, Nakamura, Akira, Akiyama, Kenichi, Aiba, Setsuya, Takai, Toshiyuki
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01-09-2003
• Subjects: Antigen presentation; Dendritic cell; Fc receptor; Humoral response; Vaccination; Dendritic cell; Antigen presentation; Fc receptor; Humoral response; Vaccination
• ISSN: 0008-8749; 1090-2163
• DOI: 10.1016/j.cellimm.2003.09.008

It is well established that activating-type Fc receptors for IgG (FcγR), such as FcγRI and FcγRIII, are essential for inducing inflammatory responses, whereas a unique inhibitory FcγR, FcγRIIB, inhibits intracellular signaling upon ligation of IgG-immune complexes, and can suppress inflammation and autoimmunity. Although antigen presentation is a crucial step for evoking inflammatory responses, the contribution of FcγRIIB to antigen presentation is controversial as to whether it regulates antigen-presenting cells (APC), particularly dendritic cells (DC), positively or negatively. In the present report, we show that the antigen targeting to both activating-type FcγRs, FcγRI/III, and inhibitory FcγRIIB on bone marrow-derived DC and macrophages and primary epidermal Langerhans’ cells augmented T cell proliferation in vitro and elicited humoral responses upon adoptive transfer of the antigen-pulsed DC. The DC lacking FcγRIIB showed a reduction in IC-uptake ability and a decreased T-cell stimulation, and induced less efficient IgG production than those of DC from wild-type mice. On the other hand, the DC lacking FcR common γ subunit, which only expresses FcγRIIB, showed significant up-regulations of IC-uptake, T-cell proliferation, and IgG production compared to those of FcγR null DC, demonstrating a positive regulation of FcγRIIB for the efficient antigen presentation of IgG-complexed antigens. These results support the therapeutic benefits of antigen-targeting to FcγR on APC in the various inflammatory disorders.