Stimulation and inhibition of secretion by phorbol myristate acetate in different cell types

• Published in: Biochemical and biophysical research communications Vol. 127; no. 3; p. 950
• Main Authors: Blackwell, G J, Bonser, R W, Dawson, J, Garland, L G
• Format: Journal Article
• Language: English
• Published: United States 29-03-1985
• Subjects: Animals; Blood Platelets - drug effects; Blood Platelets - metabolism; Calcimycin - pharmacology; Cell Line; Drug Interactions; Granulocytes - drug effects; Granulocytes - metabolism; Histamine Release - drug effects; Humans; Lysosomes - enzymology; Mast Cells - drug effects; Mast Cells - metabolism; N-Formylmethionine Leucyl-Phenylalanine - pharmacology; Phorbols - pharmacology; Rats; Serotonin - metabolism; Superoxides - metabolism; Tetradecanoylphorbol Acetate - pharmacology
• ISSN: 0006-291X
• DOI: 10.1016/S0006-291X(85)80036-X

In washed human platelets and in HL60 granulocytes phorbol myristate acetate (PMA, 1-2000nM) synergised with threshold concentrations of secretogogues to induce a sustained maximum secretory response. Likewise, superoxide production from HL60 cells maintained a maximal response at PMA concentrations between 30-300nM. At concentrations up to 10nM PMA also augmented calcium ionophore, A23187, stimulated histamine release from rat peritoneal mast cells. However, in the mast cell PMA concentrations above 10nM reduced maximum histamine release in a dose-dependent manner.