A phagocytosis capacity assay: parallel measurement of the phagocytosis and the intracellular killing in granulocytes and the influence of some substances on these processes

• Published in: Zentralblatt für Bakteriologie Vol. 275; no. 2; p. 248
• Main Authors: Süss, J, Egerer, R, Stelzner, A
• Format: Journal Article
• Language: English
• Published: Germany 01-06-1991
• Subjects: Candida albicans - immunology; Cisplatin - pharmacology; Endocytosis - immunology; Endocytosis - physiology; Granulocytes - immunology; Humans; Leucine - analogs & derivatives; Leucine - pharmacology; Naphthoquinones - pharmacology; Oxazoles - pharmacology; Phagocytosis - drug effects; Phagocytosis - immunology; Phagocytosis - physiology; Polyethylene Glycols; Saccharomyces cerevisiae - immunology; Selenomethionine - metabolism
• ISSN: 0934-8840
• DOI: 10.1016/S0934-8840(11)80072-1

A radiometric technique is described for the assessment of phagocytosis and killing of viable yeast cells by granulocytes. This technique does not require separation of extra- and intracellular microorganisms. In this method the phagocytes which contain viable yeast cells (Saccharomyces cerevisiae and Candida albicans) were disrupted by Triton X-100, and only the remaining yeast cells were isotope-labelled. The uptake of [75 Se]L-selenomethionine was used to measure the killing ability of phagocytes. This method is recommended to measure the influence of biological and pharmacological agents to ingest and kill leucocytes in vitro. The following substances affected phagocytosis and killing: Granatomycin C (decreased phagocytosis), cis-DDP (no influence), bestatin (stimulation of phagocytosis) and Z 190/69-HCl (oxazole) (stimulation of phagocytosis and killing).