p53 in biopsies of oral squamous cell carcinoma. A comparative study with a malignancy grading system

• Published in: Oral oncology Vol. 33; no. 1; pp. 5 - 9
• Main Authors: De Araújo, V.C., Loyola, A.M., Pinto, D.D. Santos, Borra, R.C., De Araújo, N.S.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 1997
• Subjects: Aged; Aged, 80 and over; Biomarkers, Tumor - metabolism; Biopsy; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - pathology; Female; Humans; Immunohistochemistry; Male; malignancy grading system; Middle Aged; Mouth Neoplasms - metabolism; Mouth Neoplasms - pathology; Neoplasm Proteins - metabolism; oral cancer; p53 protein; Tumor Suppressor Protein p53 - metabolism; oral cancer; p53 protein; malignancy grading system
• ISSN: 1368-8375; 1879-0593
• DOI: 10.1016/S0964-1955(96)00055-3

The relationship between the histological grading of malignancy and p53 protein expression was studied in 40 biopsies of oral squamous cell carcinomas. An immunohistochemical analysis was carried out using the steptavidin method preceded by a treatment with citric acid in a microwave oven. All cases were classified according to the histological malignancy grading system proposed by Anneroth
et al. (Scandinavian Journal of Dental Research 1987, 95, 229–249). The expression of p53 was found in 62.5% of the carcinomas studied. Positivity of p53 staining showed a correlation with the histological grade of malignancy and with the degree of keratinisation, nuclear polymorphism and number of mitoses.