Azacytidine plus verapamil induces the differentiation of a newly characterized biphenotypic human myeloid-B lymphoid leukemic cell line BW-90

The biphenotypic cell line BW-90 was established from the peripheral blood of a a patient with a refractory acute myelomonocytic leukemia. All cells were HLADr +, CD34 −. Dual color flow cytometry showed simultaneous expression of myeloid (CD33) and B-lymphoid surface markers (CD19) on 60% of cells,...

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Published in:	Leukemia research Vol. 22; no. 8; pp. 677 - 685
Main Authors:	Zinzar, Svetlana, Silverman, Lewis R, Richardson, Eric B, Bekesi, George, Holland, James F
Format:	Journal Article
Language:	English
Published:	Oxford Elsevier Ltd 01-08-1998 Elsevier Science
Subjects:	5-Azacytidine Antineoplastic agents Azacitidine - pharmacology Biological and medical sciences Biphenotypic Cell Differentiation - drug effects Chemotherapy Cytokines Differentiation Drug Synergism Growth Substances - pharmacology Humans Immunophenotyping Leukemia, Myelomonocytic, Acute - immunology Leukemia, Myelomonocytic, Acute - pathology Medical sciences Myeloid-B lymphoid Pharmacology. Drug treatments Recombinant Proteins - pharmacology Verapamil Verapamil - pharmacology ITS, insulin–transferrin–sodium selenite RA, all- trans-retinoic acid AcP, acid phosphatase TNF α, tumor necrosis factor alpha IL-6, interleukin 6 ICAM-1, intracellular adhesion molecule RAEB, refractory anemia with excess of blasts Biphenotypic 5AzaC, 5-azacytidine AcP+T, AcP+tartrate IL-1 α, interleukin 1 α PHA-LCM, phytohemagglutinin-stimulated lymphocyte conditioned medium G-CSF, granulocyte colony-stimulating factor IFN α, interferon-alpha 5-Azacytidine RAEB-T, refractory anemia with excess of blasts in transformation Cytokines TGF β1, transforming growth factor beta-1 AML, acute myeloid leukemia ALL, acute lymphoid leukemia IL-4, interleukin 4 AMML acute myelomonocytic leukemia GM-CSF, granulocyte-monocyte colony-stimulating factor IL-3, interleukin 3 IMDM, Iscove's modified Dulbecco medium CCM, cell-conditioned medium Myeloid-B lymphoid IL-7, interleukin 7 VP, verapamil Verapamil DMSO, dimethyl sulfoxide Differentiation Epo, erythropoietin FCS, fetal calf serum PMA, phorbol 12-myristate 13-acetate Antineoplastic agent Immunohistochemistry Cell culture Flow cytometry Calcium antagonist Myelodysplastic syndrome Myeloproliferative syndrome Azanucleoside Azacitidine Established cell line Aralkylamine Phenotypic hybrid Human Myelomonocytic leukemia Acute Treatment efficiency Cytokine Malignant hemopathy B-Lymphocyte Cell differentiation In vitro Pathology Antimetabolic Triazine derivatives Cytogenetics
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