Reliability of heterophyid antigens in heterologous protection against human schistosomiasis

• Published in: Journal of parasitic diseases Vol. 44; no. 2; pp. 349 - 354
• Main Authors: Hegazy, Alaa H. A., Galal, Lamia A., Hassan, Tasneem M., Khalifa, Refaat M. A.
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01-06-2020; Springer Nature B.V
• Subjects: Antigens; Gel electrophoresis; Health Promotion and Disease Prevention; Hyperimmunization; Immunogenicity; Infectious Diseases; Medicine; Medicine & Public Health; Original; Original Article; Parasites; Parasitic diseases; Polyacrylamide; Praziquantel; Schistosomiasis; Sodium lauryl sulfate; Western blotting; Schistosomiasis; SDS-PAGE; Western blotting; Heterologous immunity; Pygidiopsis genata
• ISSN: 0971-7196; 0975-0703
• DOI: 10.1007/s12639-020-01195-8

Schistosomiasis is a neglected tropical parasitic disease which has been controlled by praziquantel for many decades; however, reemergence of praziquantel resistant strains has been a continuous threat. Therefore, the development of reliable antischistosomal vaccine is significantly demanded for optimal control. In the present study, comparison among
Schistosoma haematobium, Schsitosoma mansoni
and
Pygidiopsis genata
crude antigens was carried out by Sodium dodecyl sulphate polyacrylamide gel electrophoresis. Hyperimmunization of rabbits with tested parasites’ crude antigens was done to obtain hyperimmune sera. Western blotting was applied to show cross reactivity between parasites’ crude antigens and either homologous or heterologous sera. Although there was no cross reaction between
P
.
genata
crude antigens and sera of both
Schistosoma
species and vice versa; it is supposed that the immunogenic band at 79 kDa might develop cross reactivity with
Schistosma
spp. SEA fractionation if used in future studies.