Stable high-copy-number bacteriophage λ promoter vectors for overproduction of proteins in Escherichia coli

The construction of new high-copy-number (hcn) λ-promoter expression vectors is described. All these vectors (1) contain tandem λ p R and p L promoters upstream of an extensive multiple cloning site (MCS) for insertion of genes, (2) direct expression of the λcI ts857 gene, enabling their use in any...

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Bibliographic Details
Published in:	Gene Vol. 176; no. 1; pp. 49 - 53
Main Authors:	Love, Christopher A., Lilley, Penelope E., Dixon, Nicholas E.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 17-10-1996
Subjects:	Bacterial Proteins - genetics Bacteriophage lambda - genetics Base Sequence DNA, Viral Escherichia coli Escherichia coli - metabolism Expression vector Gene cloning Gene Dosage Gene Expression Gene II protein Genetic Vectors Hemeproteins - genetics HMP protein Molecular Sequence Data Phage lambda Plasmids Promoter Regions, Genetic A RBS SDS SSB nt HMP protein HMP PAGE Gene II protein bp Ap p hcn LB kb Pollk Gene cloning NTA ENase Phage lambda MCS Expression vector ts
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