Effect of recombinant divercin RV41, structural variants and the activators of potassium channels on Listeria monocytogenes EGDe

• Published in: Folia microbiologica Vol. 56; no. 2; pp. 110 - 115
• Main Authors: Rihakova, J., Prevost, H., Demnerova, K., Drider, D.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-03-2011; Springer Nature B.V; Springer Verlag (Germany)
• Subjects: Adenosine Triphosphate - metabolism; Anti-Bacterial Agents - metabolism; Applied Microbiology; ATP; Bacteriocins - genetics; Bacteriocins - metabolism; Biomedical and Life Sciences; Environmental Engineering/Biotechnology; Genetic recombination; Genotype & phenotype; Immunology; Life Sciences; Listeria monocytogenes; Listeria monocytogenes - drug effects; Listeria monocytogenes - genetics; Listeria monocytogenes - growth & development; Listeria monocytogenes - metabolism; Microbial Sensitivity Tests; Microbiology; Mode of action; Potassium; Potassium Channel Blockers - metabolism; Potassium Channels - agonists; Potassium Channels - metabolism; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Pinacidil; Glipizide; Isogenic Strain; Cromakalim; Iberiotoxin
• ISSN: 0015-5632; 1874-9356
• DOI: 10.1007/s12223-011-0024-2

The effect of recombinant divercin RV41 (DvnRV41) and its structural variants on the K-channel formation was determined. The growth of
Listeria monocytogenes
EGDe (sensitive phenotype) and its isogenic strain (resistant phenotype) was assessed in the presence of DvnRV41 combined or not with pinacidil, NS1619, cromakalim (as K-channel activators), iberiotoxin and glipizide (as K-channel blockers). The combined action of DvnRV41 and K activators permitted formation of ATP-dependent pores. The combination of DvnRV41 and ATP-dependent pore activator cromakalim inhibited the growth of sensitive strain. The antilisterial activity of structural variants was less important than that of DvnRV41 but their mode of action remained overall similar.