Regional expression and subcellular localization of the tyrosine-specific phosphatase SH-PTP2 in the adult human nervous system

• Published in: Neuroscience Vol. 71; no. 4; pp. 1037 - 1042
• Main Authors: Reeves, S.A., Ueki, K., Sinha, B., Difiglia, M., Louis, D.N.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 01-04-1996
• Subjects: Adult; Blotting, Western; brain; Brain - cytology; Brain - enzymology; expression; human; Humans; Immunohistochemistry; Intracellular Signaling Peptides and Proteins; Microscopy, Electron; neurons; Neurons - enzymology; Neurons - ultrastructure; Protein Tyrosine Phosphatase, Non-Receptor Type 11; Protein Tyrosine Phosphatase, Non-Receptor Type 6; Protein Tyrosine Phosphatases - analysis; PTPID; SH-PTP2; SH2 Domain-Containing Protein Tyrosine Phosphatases; Spinal Cord - cytology; Spinal Cord - enzymology; PBS; expression; HBS-T; HEPES; PTPID; brain; SH2; human; neurons; PTP; SH-PTP2
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/0306-4522(95)00491-2

The protein tyrosine phosphatase SH-PTP2 has been implicated in a variety of cell signaling cascades, including those mediating neuronal survival. We therefore investigated the expression of SH-PTP2 in the adult human nervous system using Western blotting, immunohistochemistry and immunoelectron microscopy. SH-PTP2 immunoreactivity was noted only in neurons, but was not restricted to a specific neuronal type or location. Immunohistochemistry showed perikaryal staining, whereas Western blotting and ultrastructural analysis suggested that SH-PTP2 is present in axons as well. While immunohistochemistry showed a Nissl-like pattern in large motor neurons, immunoelectron microscopy demonstrated a diffuse pattern of cytoplasmic staining, without apparent preferential localization.
The presence of the SH2 domain-containing tyrosine-specific phosphatase SH-PTP2 in diverse neurons in the adult nervous system suggests that SH-PTP2 may play a role in a broad spectrum of neuronal responses.