A new strategy for improving cytotoxicity of a copper complex toward metastatic melanoma cells unveiled by EPR spectroscopy

• Published in: RSC advances Vol. 13; no. 14; pp. 9715 - 9719
• Main Authors: Vieira, Eduardo Guimarães, Fazzi, Rodrigo Boni, Martins, Daniel O. T. A, Sheveleva, Alena M, Tuna, Floriana, da Costa Ferreira, Ana Maria
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 20-03-2023; The Royal Society of Chemistry
• Subjects: Chemistry; Continuous radiation; Copper compounds; Electron paramagnetic resonance; Polyhedral oligomeric silsesquioxane; Spectroscopy; Toxicity
• ISSN: 2046-2069; 2046-2069
• DOI: 10.1039/d2ra07266a

A novel strategy of improving cytotoxicity against metastatic melanoma cells using an oxindolimine copper(
ii
) complex immobilized and dimerized on a modified Polyhedral Oligomeric Silsesquioxane (POSS) matrix was developed, as revealed by electron paramagnetic resonance (EPR) spectroscopy. An assured correlation between continuous-wave (CW) and pulsed EPR spectroscopies provided a complete characterization of the actual active species, its coordination environment, as well as the efficiency/selectivity of the bioconjugate materials.
An oxindolimine-copper(
ii
) complex with antitumor properties was immobilized in a silica matrix, and verified to be more active and selective due the formation of a dinuclear species, unveiled by continuous wave and pulsed EPR spectroscopy.