Constrained peptide libraries as a tool for finding mimotopes

• Published in: Gene Vol. 151; no. 1-2; p. 115
• Main Authors: McConnell, S J, Kendall, M L, Reilly, T M, Hoess, R H
• Format: Journal Article
• Language: English
• Published: Netherlands 30-12-1994
• Subjects: Amino Acid Sequence; Angiotensin II - chemistry; Angiotensin II - genetics; Angiotensin II - immunology; Antibodies, Monoclonal; Cloning, Molecular; Cosmids; Gene Library; Molecular Sequence Data; Peptides - chemistry; Peptides - immunology
• ISSN: 0378-1119
• DOI: 10.1016/0378-1119(94)90640-8

The monoclonal antibody (mAb) KAA8 recognizes the peptide angiotensin II (AII). The KAA8 mAb was biopanned using two phage-displayed peptide libraries, one unconstrained, the other constrained by a disulfide bond. After several cycles of biopanning, both libraries showed enrichment for phage that bind KAA8. Phage isolated from the unconstrained library contain a consensus sequence that matches the sequence of AII. A consensus sequence was also identified from the constrained library that does not resemble the AII sequence, and represents a mimotope of AII. We have also demonstrated that monovalent phage display can be used to discriminate between modest and high-affinity binding peptides.