Effect of PrP genotype and route of inoculation on the ability of discriminatory Western blot to distinguish scrapie from sheep bovine spongiform encephalopathy

• Published in: Journal of general virology Vol. 93; no. Pt 2; pp. 450 - 455
• Main Authors: Migliore, Sergio, Esposito, Elena, Pirisinu, Laura, Marcon, Stefano, Di Bari, Michele, D’Agostino, Claudia, Chiappini, Barbara, Conte, Michela, Sezzi, Erminia, De Grossi, Luigi, Agrimi, Umberto, Vaccari, Gabriele, Nonno, Romolo
• Format: Journal Article
• Language: English
• Published: Reading Society for General Microbiology 01-02-2012
• Subjects: Animals; Biological and medical sciences; Blotting, Western - methods; bovine spongiform encephalopathy; Cattle; Clinical Laboratory Techniques - methods; Encephalopathy, Bovine Spongiform - diagnosis; European Union; Fundamental and applied biological sciences. Psychology; Genotype; Microbiology; Miscellaneous; Non conventional transmissible agents; Prions - analysis; Prions - genetics; scrapie; Scrapie - diagnosis; Sheep; Techniques used in virology; Veterinary Medicine - methods; Virology; Western blotting; Prion disease; Nervous system diseases; Scrapie; Genotype; Bovine spongiform encephalopathy; Cerebral disorder; Infection; Vertebrata; Mammalia; Central nervous system disease; Immunoblotting assay; Sheep; Degenerative disease; Prion; Artiodactyla; Spongiform encephalopathy; Veterinary; Ungulata
• ISSN: 1465-2099; 0022-1317; 1465-2099
• DOI: 10.1099/vir.0.035469-0

Procedures for discriminating scrapie from bovine spongiform encephalopathy (BSE) in sheep are relevant to ascertain whether BSE has entered the sheep population. This study was aimed at investigating whether the suitability of an official EU discriminative method is affected by the sheep PrP genotype and route of infection.