Identification of NUP98 abnormalities in acute leukemia: JARID1A (12p13) as a new partner gene

• Published in: Genes chromosomes & cancer Vol. 45; no. 5; pp. 437 - 446
• Main Authors: van Zutven, Laura J. C. M., Önen, Emine, Velthuizen, Sandra C. J. M., van Drunen, Ellen, von Bergh, Anne R. M., van den Heuvel-Eibrink, Marry M., Veronese, Angelo, Mecucci, Cristina, Negrini, Massimo, de Greef, Georgine E., Beverloo, H. Berna
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01-05-2006
• Subjects: Acute Disease; Adolescent; Adult; Aged; Amino Acid Sequence; Base Sequence; Child; Child, Preschool; Chromosomes, Human, Pair 12; DNA Primers; Female; Humans; In Situ Hybridization, Fluorescence; Infant; Karyotyping; Leukemia - genetics; Male; Middle Aged; Molecular Sequence Data; Nuclear Pore Complex Proteins - genetics
• ISSN: 1045-2257; 1098-2264
• DOI: 10.1002/gcc.20308

Chromosome rearrangements are found in many acute leukemias. As a result, genes at the breakpoints can be disrupted, forming fusion genes. One of the genes involved in several chromosome aberrations in hematological malignancies is NUP98 (11p15). As NUP98 is close to the 11p telomere, small translocations might easily be missed. Using a NUP98‐specific split‐signal fluorescence in situ hybridization (FISH) probe combination, we analyzed 84 patients with acute myeloid leukemia (AML), acute lymphoblastic leukemia, or myelodysplastic syndrome with either normal karyotypes or 11p abnormalities to investigate whether there are unidentified 11p15 rearrangements. Neither NUP98 translocations nor deletions were identified in cases with normal karyotypes, indicating these aberrations may be very rare in this group. However, NUP98 deletions were observed in four cases with unbalanced 11p aberrations, indicating that the breakpoint is centromeric of NUP98. Rearrangements of NUP98 were identified in two patients, both showing 11p abnormalities in the diagnostic karyotype: a t(4;11)(q1?3;p15) with expression of the NUP98–RAP1GDS1 fusion product detected in a 60‐year‐old woman with AML‐M0, and an add(11)(p15) with a der(21)t(11;21)(p15;p13) observed cytogenetically in a 1‐year‐old boy with AML‐M7. JARID1A was identified as the fusion partner of NUP98 using 3′ RACE, RT‐PCR, and FISH. JARID1A, at 12p13, codes for retinoblastoma binding protein 2, a protein implicated in transcriptional regulation. This is the first report of JARID1A as a partner gene in leukemia. © 2006 Wiley‐Liss, Inc.