Maribacter antarcticus sp. nov., a psychrophilic bacterium isolated from a culture of the Antarctic green alga Pyramimonas gelidicola

• Published in: International journal of systematic and evolutionary microbiology Vol. 59; no. 6; pp. 1455 - 1459
• Main Authors: Zhang, Gwang Il, Hwang, Chung Yeon, Kang, Sung-Ho, Cho, Byung Cheol
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01-06-2009; Society for General Microbiology
• Subjects: Antarctic Regions; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; Chlorophyta - growth & development; Chlorophyta - microbiology; Culture Media; DNA, Bacterial - analysis; DNA, Ribosomal - analysis; Fatty Acids - analysis; Flavobacteriaceae - classification; Flavobacteriaceae - genetics; Flavobacteriaceae - isolation & purification; Fundamental and applied biological sciences. Psychology; Marine; Microbiology; Molecular Sequence Data; Nucleic Acid Hybridization; Phenotype; Phylogeny; Polymerase Chain Reaction - methods; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Species Specificity; Systematics; Antarctica; Antarctic Regions; Bacteria; New species; Psychrophily; Taxonomy; Algae
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.006056-0

1 School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea
2 Division of Polar Biology and Ocean Sciences, Korea Polar Research Institute (KOPRI), Get-Pearl Tower, Songdo Technopark, 7-50 Songdo-dong, Yeonsu-gu, Incheon 406-840, Republic of Korea
Correspondence Byung Cheol Cho bccho{at}snu.ac.kr
A psychrophilic, Gram-negative, dark orange-pigmented bacterium, designated CL-AP4 T , was isolated from a culture of the green alga Pyramimonas gelidicola obtained from the Southern Ocean. Strain CL-AP4 T grew optimally at 10 °C, in the presence of 3–4 % sea salts and at pH 8. 16S rRNA gene sequence analysis revealed that strain CL-AP4 T belonged to the family Flavobacteriaceae , with Maribacter arcticus KOPRI 20941 T as its closest relative (97.2 % similarity). A number of chemotaxonomic characteristics supported affiliation of strain CL-AP4 T with the genus Maribacter , i.e. iso-C 15 : 0 (17.2 %), iso-C 15 : 1 (16.8 %) and iso-C 17 : 0 3-OH (14.9 %) were the dominant fatty acids, MK-6 was the major menaquinone and the DNA G+C content was 37.1 mol%. DNA–DNA relatedness between CL-AP4 T and M. arcticus KOPRI 20941 T was only 10 %, suggesting that they are genomically distinct species. In addition, strain CL-AP4 T differed phenotypically from M. arcticus in its optimum growth temperature, its ability to hydrolyse starch, Tween 40 and Tween 80, and production of certain enzymes. On the basis of the results of the polyphasic analysis, strain CL-AP4 T was classified in the genus Maribacter as belonging to a novel species, for which the name Maribacter antarcticus sp. nov. is proposed; the type strain is CL-AP4 T (=KCCM 90069 T =JCM 15445 T ).
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CL-AP4 T is EU512921 .
A supplementary table showing the cellular fatty acid contents of strain CL-AP4 T and related species in the genus Maribacter is available with the online version of this paper.