Effect of Methionine Sulfoximine on Nitrogen Metabolism and Externally Supplied Ammonium Assimilation in Kidney Bean

• Published in: Ecotoxicology and environmental safety Vol. 34; no. 1; pp. 70 - 75
• Main Authors: Sadunishvili, T., Gvarliani, N., Nutsubidze, N., Kvesitadze, G.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01-06-1996; Elsevier
• Subjects: ACIDE AMINE; ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; Agrochemicals products; Amino Acid Oxidoreductases - metabolism; AMINO ACIDS; Amino Acids - metabolism; AMINOACIDOS; Ammonium Sulfate - metabolism; ANTIBIOTICOS; ANTIBIOTICS; ANTIBIOTIQUE; Biological and medical sciences; ENZIMAS; ENZYME; ENZYME INHIBITORS; ENZYMES; ENZYMIC ACTIVITY; Fabaceae - drug effects; Fabaceae - metabolism; Ferredoxins - metabolism; FEUILLE; FREE AMINO ACIDS; Fundamental and applied biological sciences. Psychology; GLUTAMATE AMMONIA LIGASE; Glutamate Dehydrogenase - metabolism; GLUTAMINA SINTETASA; GLUTAMINE SYNTHETASE; Glutathione Synthase - antagonists & inhibitors; HOJAS; INHIBICION; INHIBIDORES DE ENZIMAS; INHIBITEUR D'ENZYME; INHIBITION; Isotope Labeling; Kinetics; LEAVES; METABOLISME DE L'AZOTE; METABOLISMO DEL NITROGENO; Methionine Sulfoximine - metabolism; Methionine Sulfoximine - toxicity; NAD - metabolism; NITROGEN METABOLISM; Other agrochemicals used in plant protection (herbicides, fungicides, etc.); PHASEOLUS VULGARIS; Phytopathology. Animal pests. Plant and forest protection; Plant Leaves - enzymology; Plant Roots - enzymology; Plants, Medicinal; PLANTULAS; PLANTULE; Pollution effects and side effects of agrochemicals on crop plants and forest trees. Other anthropogenic factors; Pollution effects. Side effects of agrochemicals; Quaternary Ammonium Compounds - metabolism; RACINE; RAICES; ROOTS; SEEDLINGS; Assimilation; Ammonium; Carbon-nitrogen ligases; Enzyme; Enzyme inhibitor; Nitrogen; Metabolism; Grain legume; Leguminosae; Ligases; Phaseolus vulgaris; Aminoacid; Dicotyledones; Angiospermae; Spermatophyta; Inhibition; Glutamate-ammonia ligase
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1006/eesa.1996.0046

L-Methionine sulfoximine (MSO) at concentration 1.25 mM in vivocauses the inhibition of glutamine synthetase (GS) in both roots and leaves of young seedlings of kidney bean following the accumulation of high levels of ammonia and decrease in amounts of free amino acids that is more pronounced in leaves. The inhibition of GS by MSO in leaves in the case of externally supplied 5 mM(15NH4)2SO4assimilation leads to ammonia accumulation and the decrease in the amounts of glutamine and glutamic acid and the intensity of the incorporation of15N into them. In roots the inhibition of GS is not followed by the decrease of15N content into glutamate. It is concluded that the pathway of ammonia primary assimilation in leaves is via GS and glutamate synthase (GOGAT), while in roots glutamate dehydrogenase also plays an important role in this process.