Functional Properties of Lactobacillus mucosae Strains Isolated from Brazilian Goat Milk

• Published in: Probiotics and antimicrobial proteins Vol. 9; no. 3; pp. 235 - 245
• Main Authors: de Moraes, Georgia Maciel Dias, de Abreu, Louricélia Rodrigues, do Egito, Antônio Silvio, Salles, Hévila Oliveira, da Silva, Liana Maria Ferreira, Nero, Luís Augusto, Todorov, Svetoslav Dimitrov, dos Santos, Karina Maria Olbrich
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-09-2017
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Applied Microbiology; Bacterial Adhesion - genetics; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bile Acids and Salts - metabolism; Biogenic Amines - biosynthesis; Brazil; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Drug Resistance, Multiple, Bacterial; Food Microbiology; Gastrointestinal Tract - metabolism; Gastrointestinal Tract - microbiology; Gelatinases - biosynthesis; Genes, Bacterial; Goats; Hydrophobic and Hydrophilic Interactions; Lactobacillus - genetics; Lactobacillus - isolation & purification; Lactobacillus - physiology; Microbiology; Milk - microbiology; Nutrition; Probiotics - isolation & purification; Protein Science; Virulence Factors - genetics; Lactic acid bacteria; Probiotic potential; Technological properties; LAB; Virulence genes; Lactobacillus mucosae
• ISSN: 1867-1306; 1867-1314
• DOI: 10.1007/s12602-016-9244-8

The search for probiotic candidates among lactic acid bacteria (LAB) isolated from food may uncover new strains with promising health and technological properties.
Lactobacillus mucosae
strains attracted recent research attention due to their ability to adhere to intestinal mucus and to inhibit pathogens in the gastrointestinal tract, both related to a probiotic potential. Properties of interest and safety aspects of three
Lb. mucosae
strains (CNPC006, CNPC007, and CNPC009) isolated from goat milk were investigated employing in vitro tests. The presence of genetic factors related to bile salt hydrolase production (
bsh
), intestinal adhesion properties (
msa
,
map
,
mub
, and
ef-tu
), virulence, and biogenic amine production were also verified. All strains exhibited the target
map
,
mub
, and
ef-tu
sequences; the
msa
gene was detected in CNPC006 and CNPC007 strains. Some of the searched sequences for virulence factors were detected, especially in the CNPC009 strain; all strains carried the
hyl
gene, related to the production of hyaluronidase.
Lb. mucosae
CNPC007 exhibited a high survival rate in simulated gastric and enteric conditions. Besides, all strains exhibited the
bsh
sequence, and CNPC006 and CNPC007 were able to deconjugate salts of glycodeoxycholic acid (GDC). Regarding technological properties for dairy product applications, a relatively higher milk acidification and clotting capacity, diacetyl production, and proteolytic activity were registered for CNPC007 in comparison to the other strains. Collectively, the results aim at
Lb. mucosae
CNPC007 as a promising probiotic candidate for application in dairy products, deserving further studies to confirm and explore its potential.