Organophilic treatments of bentonite increase the adsorption of aflatoxin B1 and protect stem cells against cellular damage

[Display omitted] •Organophilic treatments of bentonite increased AFB1 adsorption.•Concentration and temperature affect the organophilic treatments of bentonite.•The organic bentonites did not cause toxicity in neural crest stem cells.•The new materials protected the stem cells against damage caused...

Full description

Saved in:
Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces Vol. 145; pp. 555 - 561
Main Authors: Nones, Janaína, Nones, Jader, Poli, Anicleto, Trentin, Andrea Gonçalves, Riella, Humberto Gracher, Kuhnen, Nivaldo Cabral
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-09-2016
Subjects:
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:[Display omitted] •Organophilic treatments of bentonite increased AFB1 adsorption.•Concentration and temperature affect the organophilic treatments of bentonite.•The organic bentonites did not cause toxicity in neural crest stem cells.•The new materials protected the stem cells against damage caused by AFB1.•BAC and CTAB offer new strategies against aflatoxicoses in animals and humans. Bentonite clays exhibit high adsorptive capacity for contaminants, including aflatoxin B1 (AFB1), a mycotoxin responsible for causing severe toxicity in several species including pigs, poultry and man. Organophilic treatments is known to increase the adsorption capacity of bentonites, and the primary aim of this study was to evaluate the ability of Brazilian bentonite and two organic salts - benzalkonium chloride (BAC) and cetyltrimethylammonium bromide (CTAB) to adsorb AFB1. For this end, 22 factorial designs were used in order to analyze if BAC or CTAB was able to increase AFB1 adsorption when submitted in different temperature and concentration. Both BAC and CTAB treatment (at 30°C and 2% of salt concentration) were found to increase the adsorption of AFB1 significantly compared with untreated bentonite. After organophilic bentonite treatments with BAC or CTAB, a vibration of CH stretch (2850 and 2920cm−1) were detected. A frequency of the SiO stretch (1020 and 1090cm−1) was changed by intercalation of organic cation. Furthermore, the interlayer spacing of bentonite increases to 1.23nm (d001 reflection at 2θ=7.16) and 1.22 (d001 reflection at 2θ=7.22) after the addition of BAC and CTAB, respectively. Another aim of the study was to observe the effects of these two bentonite salts in neural crest stem cell cultures. The two materials that were created by organophilic treatments were not found to be toxic to stem cells. Furthermore the results indicate that the two materials tested may protect the neural crest stem cells against damage caused by AFB1.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2016.05.061