A lectin with antifungal activity from the mussel Crenomytilus grayanus

• Published in: Fish & shellfish immunology Vol. 42; no. 2; pp. 503 - 507
• Main Authors: Chikalovets, Irina V., Chernikov, Oleg V., Pivkin, Mikhail V., Molchanova, Valentina I., Litovchenko, Alina P., Li, Wei, Lukyanov, Pavel A.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-02-2015
• Subjects: Animals; Antifungal activity; Antifungal Agents - metabolism; Crenomytilus grayanus; Galanin - genetics; Galanin - metabolism; GalNAc/Gal-specific lectin; Innate immunity; Lectin expression; Lectins - genetics; Lectins - metabolism; Marine; Mollusca; Mytilidae - genetics; Mytilidae - immunology; Mytilidae - metabolism; Organ Specificity; Pichia - physiology; Pichia pastoris; Lectin expression; Antifungal activity; Crenomytilus grayanus; Innate immunity; GalNAc/Gal-specific lectin
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2014.11.036

Lectins (carbohydrate-binding proteins) are well known to actively participate in the defense functions of vertebrates and invertebrates where they play an important role in the recognition of foreign particles. In this study, we investigated of in vitro antifungal activity of lectin from the mussel Crenomytilus grayanus (CGL). Enzyme-linked immunosorbent assay indicated that CGL was predominantly detectable in tissues of mantle and to a lesser degree in the tissues of muscle, hepatopancreas, gill and hemocytes. After challenged by Pichia pastoris the level of CGL was upregulated and reached the maximum level at 12 h post challenge and recovered to the original level at 24 h. The lectin was capable of inhibiting the germination of spores and hyphal growth in the fungi. All these results indicated that CGL is involved in the innate immune response in mollusc animals.
•Antifungal activity of lectin from the mussel Crenomytilus grayanus was determined.•CGL exhibited the highest expression in mantle.•The level of CGL was upregulated after Pichia pastoris challenge.•CGL was able to inhibit the in vitro growth of the fungi.•The lectin plays defensive role in the mollusk.