Serial transmission of spongiocytic hepatitis to woodchucks (possible association with a specific delta strain)

• Published in: Journal of hepatology Vol. 22; no. 4; pp. 468 - 473
• Main Authors: Parana, Raymundo, Gerard, Françoise, Lesbordes, Jean-Louis, Pichoud, Christian, Vitvitski, Ludmila, Costa Lyra, Luiz Guilherme, Trepo, Christian
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier B.V 01-04-1995; Elsevier
• Subjects: Animals; Antigen-Antibody Reactions; Antigens, Viral - analysis; Biological and medical sciences; Blood; Carrier State; Fulminant delta hepatitis; Hepatitis B Virus, Woodchuck - isolation & purification; Hepatitis D - pathology; Hepatitis D - transmission; Hepatitis D - virology; Hepatitis Delta Virus - genetics; Hepatitis Delta Virus - immunology; Human viral diseases; Humans; Infectious diseases; Injections; Liver - chemistry; Liver - immunology; Liver - pathology; Liver - virology; Marmota - virology; Medical sciences; Species Specificity; Spongiocytes; Tissue Extracts - pharmacology; Viral diseases; Viral hepatitis; Woodchucks; Spongiocytes; Fulminant delta hepatitis; Woodchucks; Human; Marmota monax; Rodentia; Hepatic disease; Infection; Pathology; Vertebrata; Inoculation; Mammalia; Viral disease; Animal; Viral hepatitis delta; Digestive diseases; Wood chuck hepatitis
• ISSN: 0168-8278; 1600-0641
• DOI: 10.1016/0168-8278(95)80111-1

Background/Aims: Outbreaks of severe hepatitis have been reported from Africa and South America. Description of the case has shown the histological hallmark to be the presence of ballooning hepatocytes with fat drops surrounding the nucleus (spongiocytes or morula cells).
Methods: Experimental reproduction of this syndrome for the verification of a possible role of a specific HDV strain was performed by the inoculation of serum and liver extracts from African patients (Bangui-Central African Republic), who died with this syndrome, into American woodchuck carriers of WHV (WC 231,144), the results of which were then compared with animals inoculated with a reference wild HDV strain (WC 300,173,154), and those which received material from a European fulminant HDV case (WC 88,93).
Results: Following the initial inoculation, the animals receiving African inocula had a delayed anti-HDV seroconversion, high mortality and showed the presence of spongiocytes, while the other animals had a classical evolution of HDV superinfection in woodchucks. Furthermore, the African inocula caused less inhibition of WHV replication, as well as a predominant cytoplasmic expression of HDAg, in contrast to the animals which received the other inocula. The second passage experiments gave similar results.
Conclusions: We conclude that this peculiar form of HDV fulminant hepatitis can be experimentally reproduced and might be specifically related to a more pathogenic strain.