An enzymatic assay for picomole levels of phosphatidate

An assay for phosphatidate that is sensitive, specific, and highly reproducible is described. Phosphatidate is transacylated with methylamine and the glycerol 3-phosphate produced is measured enzymatically. The assay is linear from 50 to 1500 pmol of phosphatidate and can be used to quantitate phosp...

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Bibliographic Details
Published in:	Analytical biochemistry Vol. 180; no. 1; p. 24
Main Authors:	Bocckino, S B, Wilson, P, Exton, J H
Format:	Journal Article
Language:	English
Published:	United States 01-07-1989
Subjects:	Animals Chromatography, High Pressure Liquid Chromatography, Thin Layer Glycerol-3-Phosphate Dehydrogenase (NAD+) Glycerolphosphate Dehydrogenase In Vitro Techniques Liver - analysis Liver - cytology Male Methylamines Microchemistry NAD Phosphatidic Acids - analysis Phosphorus Isotopes Rats
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Description
Summary:	An assay for phosphatidate that is sensitive, specific, and highly reproducible is described. Phosphatidate is transacylated with methylamine and the glycerol 3-phosphate produced is measured enzymatically. The assay is linear from 50 to 1500 pmol of phosphatidate and can be used to quantitate phosphatidate in small amounts of biological materials.
ISSN:	0003-2697
DOI:	10.1016/0003-2697(89)90083-3