Influence of continuous light treatment on expression of stress biomarkers in Atlantic cod

• Published in: Developmental and comparative immunology Vol. 44; no. 1; pp. 30 - 34
• Main Authors: Giannetto, Alessia, Fernandes, Jorge M.O., Nagasawa, Kazue, Mauceri, Angela, Maisano, Maria, De Domenico, Elena, Cappello, Tiziana, Oliva, Sabrina, Fasulo, Salvatore
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 01-05-2014
• Subjects: Adaptation, Ocular; Animals; Antimicrobial Cationic Peptides - genetics; Antimicrobial Cationic Peptides - metabolism; Antimicrobial peptides; Atlantic cod; Biomarkers - metabolism; Catalase - genetics; Catalase - metabolism; Gadus morhua; Gadus morhua - immunology; Gene expression; Gene Expression Regulation; Glutathione Reductase - genetics; Glutathione Reductase - metabolism; Light - adverse effects; Marine; Oxidative Stress; Photoperiod; Stress genes; Stress, Physiological - genetics; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; A, Atlantic; Atlantic cod; Photoperiod; Gene expression; Stress genes; Antimicrobial peptides
• ISSN: 0145-305X; 1879-0089
• DOI: 10.1016/j.dci.2013.11.011

•Genes involved in oxidative stress were differentially expressed with photoperiod.•Photoperiod modulates liver transcript levels of key antimicrobial peptides.•These transcriptional changes may be an adaptive response to light stress.
Continuous light treatment during early juvenile stages in Gadus morhua is a common farming management practice but the effects of these unnatural light conditions on fish stress have received scant attention. In the present study we investigated how continuous illumination affects transcription levels of key stress-related and antimicrobial peptide genes in juvenile Atlantic cod. Gene expression quantification by real-time PCR revealed higher levels of transcripts coding for antioxidant enzymes, namely superoxide dismutase, catalase and glutathione reductase in liver of fish reared under continuous illumination, concomitantly with a 43% decrease in glutathione content. Transcription of antimicrobial peptides such as piscidins, hepcidin and cathelicidin was also affected by constant illumination. Overall, the significant changes in liver transcript levels of these biomarkers in response to continuous light may be an adaptation to light stress.