Utilization of human cultured epidermal keratinocytes: irreversibility of the inhibition of proliferation induced in stored detached cultures

In order to look for the best conditions required for the utilization of cultured epithelium in the treatment of burn wounds, some experiments have been performed studying the storage of dispase-detached cultures. The viability of keratinocytes and, after trypsin dissociation of the cultures, the ad...

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Bibliographic Details
Published in:	Burns : journal of the International Society for Burn Injuries Vol. 17; no. 3; p. 205
Main Authors:	Boucher, F, Poumay, Y, Degen, A, Paye, M, Leloup, R
Format:	Journal Article
Language:	English
Published:	Netherlands 01-06-1991
Subjects:	Burns - surgery Cell Adhesion - physiology Cell Survival - physiology Cells, Cultured Humans Keratinocytes - transplantation Skin - cytology Skin Transplantation - methods Tissue Preservation
Online Access:	Get more information
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Summary:	In order to look for the best conditions required for the utilization of cultured epithelium in the treatment of burn wounds, some experiments have been performed studying the storage of dispase-detached cultures. The viability of keratinocytes and, after trypsin dissociation of the cultures, the adhesion and proliferative potential of living keratinocytes were measured. Our laboratory investigations suggest that the storage period of detached cultures has to be kept as short as possible to preserve the keratinocytes' growth potential.
ISSN:	0305-4179
DOI:	10.1016/0305-4179(91)90105-P