Impaired nitric oxide release by glomeruli from diabetic rats

• Published in: Metabolism, clinical and experimental Vol. 44; no. 6; pp. 695 - 698
• Main Authors: Craven, Patricia A., Studer, Rebecca K., DeRubertis, Frederick R.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01-06-1995; Elsevier
• Subjects: Animals; Biological and medical sciences; Calcimycin - pharmacology; Carbachol - pharmacology; Diabetes Mellitus, Experimental - metabolism; Diabetes. Impaired glucose tolerance; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Female; In Vitro Techniques; Kidney Glomerulus - metabolism; Medical sciences; Nitric Oxide - metabolism; Penicillamine - analogs & derivatives; Penicillamine - pharmacology; Rats; Rats, Sprague-Dawley; Reference Values; S-Nitroso-N-Acetylpenicillamine; Endocrinopathy; Renal glomerulus; Vertebrata; Metabolic disorder; Mammalia; Renal function; Pathophysiology; Rat; Animal; Diabetes mellitus; Rodentia
• ISSN: 0026-0495; 1532-8600
• DOI: 10.1016/0026-0495(95)90178-7

Basal nitric oxide (NO) production and NO responses to carbamylcholine (CCh) and the Ca
2+ ionophore A23187 were measured with a NO electrode in glomeruli isolated from 2 to 3-month-diabetic versus age-matched control rats. In the presence of CCh or A23187, NO production was markedly reduced in glomeruli from diabetic versus control rats. Spontaneous generation of NO by
s-nitrosopenicillamine (SNAP) was also reduced in the presence of glomeruli from diabetic rats as compared with values either in control glomeruli or in buffer alone. The results demonstrate an impairment of NO generation and/or stability in glomeruli from 2 to 3-month-diabetic rats, which correlates with the previously observed suppression of NO-dependent glomerular cyclic guanosine 3′,5′-monophosphate (cGMP) induced by diabetes.