Liquid chromatography/tandem mass spectrometry study of anti-inflammatory activity of Plantain ( Plantago L.) species

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 52; no. 5; pp. 701 - 706
• Main Authors: Beara, Ivana N., Orčić, Dejan Z., Lesjak, Marija M., Mimica-Dukić, Neda M., Peković, Biljana A., Popović, Mira R.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 05-09-2010; Elsevier
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Anti-inflammatory activity; Anti-Inflammatory Agents - pharmacology; Biological and medical sciences; Chromatography, Liquid - methods; Cyclooxygenase; Cyclooxygenase Inhibitors - pharmacology; Fundamental and applied biological sciences. Psychology; General pharmacology; Humans; LC–MS/MS; Lipoxygenase; Lipoxygenase Inhibitors - pharmacology; Medical sciences; Pharmacology. Drug treatments; Plant Extracts - pharmacology; Plantago; Plantago - chemistry; Platelets; Tandem Mass Spectrometry - methods; COX-2; 12-HETE; COX-1; MS2SIM; MRM; LC–MS/MS; Cyclooxygenase; Plantago; Anti-inflammatory activity; 12-LOX; 12-HHT; Lipoxygenase; Platelets; Prostaglandin-endoperoxide synthase; Enzyme; Antiinflammatory agent; HPLC chromatography; Plantaginaceae; LC-MS/MS; Platelet; Dicotyledones; Mass spectrometry MS/MS; Angiospermae; Spermatophyta; Oxidoreductases; Species
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2010.02.014

To evaluate anti-inflammatory activity of selected
Plantago species (
P. lanceolata L. and
P. major L.) an optimized
in vitro test for determination of cyclooxygenase-1 (COX-1) and 12-lipoxygenase (12-LOX) inhibition potency was undertaken. By using intact cell system (platelets) as a source of COX-1 and 12-LOX enzymes and highly sensitive and specific LC–MS/MS technique for detection of main arachidonic acid metabolites formed by COX-1 and 12-LOX, this test provides efficient method for evaluation of anti-inflammatory potential of plant extracts and isolated compounds. Our results validated the well-known COX-1 inhibitory activity of
P. lanceolata and
P. major methanol extracts (concentration required for 50% inhibition (IC
50) was 2.00 and 0.65
mg/ml, respectively). Furthermore, 12-LOX inhibitory activity of examined extracts was reported for the first time (IC
50
=
0.75 and 1.73
mg/ml for
P. lanceolata and
P. major, respectively). Although renowned inhibitors, such as acetylsalicylic acid and quercetin showed higher activity, this study verifies
P. lanceolata and
P. major as considerable anti-inflammatory agents.