Characterization of Aspergillus oryzae fermentation extract effects on the rumen fungus Neocallimastix frontalis, EB 188. Part 1. Zoospore development and physiology

• Published in: Applied microbiology and biotechnology Vol. 63; no. 4; pp. 422 - 430
• Main Authors: SCHMIDT, J. A, ALBRIGHT, S, TSAI, K.-P, CALZA, G. M, CHANG, J. S, CALZA, R. E
• Format: Journal Article
• Language: English
• Published: Berlin Springer 2004; Springer Nature B.V
• Subjects: Amylases - metabolism; Animals; Aspergillus oryzae; Aspergillus oryzae - metabolism; beta-Glucosidase - metabolism; Biological and medical sciences; Biotechnology; Carbohydrate Metabolism; Carbon sources; Cattle; Cellulase - biosynthesis; Cellulase - metabolism; Cellulose; Dairy cattle; Dietary Supplements; Enzymes; Enzymes - metabolism; Fatty Acids, Volatile - analysis; Fermentation; Fundamental and applied biological sciences. Psychology; Fungal Proteins - analysis; Fungi; L-Lactate Dehydrogenase - metabolism; Malate Dehydrogenase - metabolism; Neocallimastix - chemistry; Neocallimastix - enzymology; Neocallimastix - growth & development; Neocallimastix frontalis; Oil and gas production; Physiology; Rumen - microbiology; Spores, Fungal - chemistry; Spores, Fungal - growth & development; Wheat bran; Phycomycetes; Dairy cattle; Rumen; Extract; Fermentation; Fungi; Vertebrata; Mammalia; Aspergillus oryzae; Physiology; Artiodactyla; Fungi Imperfecti; Supplementation; Application; Ungulata; Neocallimastix; Thallophyta
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-003-1293-8

Experiments were performed to determine the effect of Aspergillus oryzae (AO) fermentation extract on zoospore development in the rumen fungus Neocallimastix frontalis EB 188. Powdered product, or liquid extract prepared from such powder, was added at the recommended value for supplementation in dairy cattle. Stationary and stirred cultures were periodically sampled and assayed for extracellular and intracellular protein and enzymes, gas production, zoospore production and maturation, and carbon source utilization. Soluble extract increased fungal physiology when grown in stirred vessels or stationary cultures. Treated cultures produced higher levels of enzymes (nearly double). Mobile zoospores matured into germination entities more rapidly in treated cultures, and when powdered product was used, nearly 3 times more motile zoospores were produced at 56 h of fungal growth. Levels of the intracellular enzyme malate dehydrogenase increased by 6-fold in the presence of powdered product. Product wheat bran carrier used as soluble extract or powder had very little effect on fungal cultures. Medium cellulose was completely hydrolyzed in all cultures but this occurred earlier in those containing AO treatment.