The complete genomic sequence of strain ROS/HUVLV-100, a representative Russian Crimean Congo hemorrhagic fever virus strain

The complete genomic sequence (minus primer-generated ends) of the laboratory-adapted Crimean Congo hemorrhagic fever virus (CCHFV) strain ROS/HUVLV-100, isolated in 2003 from the blood of a deceased female from the Rostov region of southern European Russia, was determined by direct sequencing of ov...

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Published in:Virus genes Vol. 33; no. 1; pp. 87 - 93
Main Authors: Meissner, John D, Seregin, Sergey S, Seregin, Sergei V, Vyshemirskii, Oleg I, Yakimenko, Nina V, Netesov, Sergei V, Petrov, Vladimir S
Format: Journal Article
Language:English
Published: United States Springer Nature B.V 01-08-2006
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Abstract The complete genomic sequence (minus primer-generated ends) of the laboratory-adapted Crimean Congo hemorrhagic fever virus (CCHFV) strain ROS/HUVLV-100, isolated in 2003 from the blood of a deceased female from the Rostov region of southern European Russia, was determined by direct sequencing of overlapping reverse transcription/polymerase chain reaction amplified products. The size of the ROS/HUVLV-100 genome is 19.2 kilobases--individual genome segments are similar in size and sequence features to previously reported "Europe-1" group CCHFV strains. The low-passage ROS/HUVLV-100 strain is the first Russian Crimean Congo hemorrhagic fever virus isolate for which complete sequence information is available, and this work reports the first complete genomic CCHFV sequence determined from a single viral RNA preparation in the same laboratory.
AbstractList The complete genomic sequence (minus primer-generated ends) of the laboratory-adapted Crimean Congo hemorrhagic fever virus (CCHFV) strain ROS/HUVLV-100, isolated in 2003 from the blood of a deceased female from the Rostov region of southern European Russia, was determined by direct sequencing of overlapping reverse transcription/polymerase chain reaction amplified products. The size of the ROS/HUVLV-100 genome is 19.2 kilobases—individual genome segments are similar in size and sequence features to previously reported “Europe-1” group CCHFV strains. The low-passage ROS/HUVLV-100 strain is the first Russian Crimean Congo hemorrhagic fever virus isolate for which complete sequence information is available, and this work reports the first complete genomic CCHFV sequence determined from a single viral RNA preparation in the same laboratory.
Author Meissner, John D
Petrov, Vladimir S
Vyshemirskii, Oleg I
Netesov, Sergei V
Seregin, Sergei V
Seregin, Sergey S
Yakimenko, Nina V
Author_xml – sequence: 1
  givenname: John D
  surname: Meissner
  fullname: Meissner, John D
  email: jo.meis@gmail.com
  organization: State Research Center of Virology and Biotechnology VECTOR, Koltsovo, Novosibirsk Region, 630559, Russia. jo.meis@gmail.com
– sequence: 2
  givenname: Sergey S
  surname: Seregin
  fullname: Seregin, Sergey S
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  givenname: Sergei V
  surname: Seregin
  fullname: Seregin, Sergei V
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  surname: Vyshemirskii
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  givenname: Nina V
  surname: Yakimenko
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  surname: Netesov
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  fullname: Petrov, Vladimir S
BackLink https://www.ncbi.nlm.nih.gov/pubmed/16791423$$D View this record in MEDLINE/PubMed
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CitedBy_id crossref_primary_10_1016_j_virusres_2009_11_009
crossref_primary_10_1016_j_cimid_2007_07_001
crossref_primary_10_1017_S0950268814000818
crossref_primary_10_1186_1743_422X_5_7
crossref_primary_10_3103_S0891416813040071
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Snippet The complete genomic sequence (minus primer-generated ends) of the laboratory-adapted Crimean Congo hemorrhagic fever virus (CCHFV) strain ROS/HUVLV-100,...
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StartPage 87
SubjectTerms Crimean hemorrhagic fever
Female
Genome, Viral
Genomes
Genomics
Hemorrhage
Hemorrhagic Fever Virus, Crimean-Congo - classification
Hemorrhagic Fever Virus, Crimean-Congo - genetics
Hemorrhagic Fever Virus, Crimean-Congo - isolation & purification
Humans
Infectious diseases
Laboratories
Molecular Sequence Data
Nucleotide sequence
Reverse transcription
Russia
Viruses
Title The complete genomic sequence of strain ROS/HUVLV-100, a representative Russian Crimean Congo hemorrhagic fever virus strain
URI https://www.ncbi.nlm.nih.gov/pubmed/16791423
https://www.proquest.com/docview/223670591
https://www.proquest.com/docview/2664218050
https://search.proquest.com/docview/20226995
https://search.proquest.com/docview/68571949
Volume 33
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