Mitogenic effect by lipopolysaccharide and pokeweed lectin on density-inhibited chick embryo fibroblasts

The B lymphocyte mitogens, bacterial lipopolysaccharide (LPS), pokeweed lectin, and tuberculin, induced proliferation in density-inhibited monolayer cultures of chick embryo fibroblasts. The stimulation was seen both as an early increase in sugar uptake and cell volume and later as an increase in th...

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Bibliographic Details
Published in:	The Journal of experimental medicine Vol. 138; no. 6; pp. 1356 - 1364
Main Authors:	Vaheri, A, Ruoslahti, E, Sarvas, M, Nurminen, M
Format:	Journal Article
Language:	English
Published:	United States The Rockefeller University Press 01-12-1973
Subjects:	Animals Carbohydrate Metabolism Cell Division Cell Membrane - metabolism Cells, Cultured Chick Embryo DNA - biosynthesis Fibroblasts - growth & development Lectins Lipopolysaccharides Mitogens Polysaccharides, Bacterial Thymidine - metabolism Tritium Tuberculin
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Summary:	The B lymphocyte mitogens, bacterial lipopolysaccharide (LPS), pokeweed lectin, and tuberculin, induced proliferation in density-inhibited monolayer cultures of chick embryo fibroblasts. The stimulation was seen both as an early increase in sugar uptake and cell volume and later as an increase in thymidine incorporation and cell number. The concentration of LPS maximally stimulating fibroblasts was remarkably low, about 0.1-1 ng/ml. LPS preparation with very different sugar chains gave quite similar results indicating that the architecture of the hydrophilic carbohydrate part is not critical for the mitogenic effect.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-1007 1540-9538
DOI:	10.1084/jem.138.6.1356