Development of a Genetically-Engineered, Candidate Polio Vaccine Employing the Self-Assembling Properties of the Tobacco Mosaic Virus Coat Protein
A synthetic gene coding for the coat protein of tobacco mosaic virus (TMVCP) was expressed in under the direction of the UV5 promoter. Modification of the 3' end of the TMVCP gene by insertion of a region coding for an antigenic epitope from poliovirus type 3 resulted in the production of a hyb...
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| Published in: | Bio/Technology (Nature Publishing Company) Vol. 4; no. 7; pp. 637 - 641 |
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| Main Authors: | , , , , , |
| Format: | Journal Article |
| Language: | English |
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1986
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| Abstract | A synthetic gene coding for the coat protein of tobacco mosaic virus (TMVCP) was expressed in
under the direction of the
UV5 promoter. Modification of the 3' end of the TMVCP gene by insertion of a region coding for an antigenic epitope from poliovirus type 3 resulted in the production of a hybrid TMVCP (TMVCP-polio 3). Both the
-produced TMVCP and TMVCP-polio 3 were shown to assemble into virus-like rods under acidic conditions in
extracts. Their purification was accomplished in a single step by chromatography on Sepharose 6B. TMVCP-polio 3 induced the formation of poliovirus neutralizing antibodies following injection into rats. The level of immune response was related to the degree of polymerization of the TMVCP-polio 3 preparations. |
|---|---|
| AbstractList | A synthetic gene coding for the coat protein of tobacco mosaic virus (TMVCP) was expressed in
under the direction of the
UV5 promoter. Modification of the 3' end of the TMVCP gene by insertion of a region coding for an antigenic epitope from poliovirus type 3 resulted in the production of a hybrid TMVCP (TMVCP-polio 3). Both the
-produced TMVCP and TMVCP-polio 3 were shown to assemble into virus-like rods under acidic conditions in
extracts. Their purification was accomplished in a single step by chromatography on Sepharose 6B. TMVCP-polio 3 induced the formation of poliovirus neutralizing antibodies following injection into rats. The level of immune response was related to the degree of polymerization of the TMVCP-polio 3 preparations. A synthetic gene coding for the coat protein of tobacco mosaic virus (TMVCP) was expressed in E. coli under the direction of the lac UV5 promoter. Modification of the 3′ end of the TMVCP gene by insertion of a region coding for an antigenic epitope from poliovirus type 3 resulted in the production of a hybrid TMVCP (TMVCP–polio 3). Both the E. coli –produced TMVCP and TMVCP–polio 3 were shown to assemble into virus–like rods under acidic conditions in E. coli extracts. Their purification was accomplished in a single step by chromatography on Sepharose 6B. TMVCP–polio 3 induced the formation of poliovirus neutralizing antibodies following injection into rats. The level of immune response was related to the degree of polymerization of the TMVCP–polio 3 preparations. |
| Author | Haynes, Joel R Cunningham, Janet Lennick, Michael Shen, Shi-Hsiang von Seefried, Adolph Garvin, Robert T |
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| Keywords | Immune response Antigenic determinant Rat Picornaviridae Escherichia coli Rodentia Tobamovirus Enterovirus Vaccine Gene expression Virus Vertebrata Mammalia Molecular assembly Tobacco mosaic virus Bacteria Coat protein Poliovirus 3 Escherichieae Hybrid gene Enterobacteriaceae |
| Language | English |
| License | CC BY 4.0 Nature Publishing Company 1986. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
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| References_xml | – volume: 47 start-page: 119 year: 1981 end-page: 128 ident: b25 publication-title: Dev. Biol. Stand. contributor: fullname: Sekhuis, V.M. – volume: 87 start-page: 107 year: 1978 end-page: 110 ident: b21 publication-title: FEBS Lett. contributor: fullname: Coulson, A.R. – volume: 79 start-page: 7871 year: 1982 end-page: 7875 ident: b8 publication-title: Proc. Natl. Acad. Sci. USA contributor: fullname: Strick, N. – volume: 123 start-page: 14 year: 1982 end-page: 22 ident: b24 publication-title: Analytical Biochem. contributor: fullname: Menge, A.C. – volume: 67 start-page: 315 year: 1972 end-page: 332 ident: b16 publication-title: J. Mol. Biol. contributor: fullname: Klug, A. – start-page: 393 year: 1984 end-page: 396 ident: b19 article-title: Modern Approaches to Vaccines contributor: fullname: Gordon, L.K. – volume: 79 start-page: 5818 year: 1982 end-page: 5822 ident: b13 publication-title: Proc. Natl. Acad. Sci. USA contributor: fullname: Karn, J. – volume: 22 start-page: 145 year: 1985 end-page: 154 ident: b2 publication-title: Molecular Immunology contributor: fullname: Jackson, D.C. – volume: 18 start-page: 199 year: 1982 end-page: 209 ident: b12 publication-title: Gene contributor: fullname: Fiers, W. – volume: 227 start-page: 680 year: 1970 end-page: 685 ident: b23 publication-title: Nature contributor: fullname: Laemmli, U.K. – volume: 65 start-page: 253 year: 1984 end-page: 279 ident: b11 publication-title: J. Gen. Virol. contributor: fullname: Butler, P.J.G. – volume: 124 start-page: 88 year: 1982 end-page: 91 ident: b22 publication-title: Analytical Biochem. contributor: fullname: Wilcox, G. – volume: 81 start-page: 2461 year: 1984 end-page: 2465 ident: b5 publication-title: Proc. Natl. Acad. Sci. USA contributor: fullname: Arnon, R. – volume: 3 start-page: 323 year: 1985 end-page: 326 ident: b18 publication-title: BioTechnology contributor: fullname: Graves, R.V. – volume: 21 start-page: 13 year: 1984 end-page: 16 ident: b4 publication-title: Molecular Immunology contributor: fullname: Hopp, T.P. – volume: 304 start-page: 699 year: 1983 end-page: 703 ident: b6 publication-title: Nature contributor: fullname: Wimmer, E. – volume: 80 start-page: 2365 year: 1983 end-page: 2369 ident: b7 publication-title: Proc. Natl. Acad. Sci. USA contributor: fullname: Lerner, R.A. – volume: 135 start-page: 2319 year: 1985 end-page: 2322 ident: b20 publication-title: J. Immunology contributor: fullname: Chedid, L. – volume: 19 start-page: 43 year: 1982 end-page: 54 ident: b14 publication-title: Gene contributor: fullname: Fuller, F. – volume: 304 start-page: 459 year: 1983 end-page: 462 ident: b15 publication-title: Nature contributor: fullname: Almond, J.W. – volume: 67 start-page: 307 year: 1972 end-page: 314 ident: b17 publication-title: J. Mol. Biol. contributor: fullname: Finch, J.T. – volume: 82 start-page: 910 year: 1985 end-page: 914 ident: b1 publication-title: Proc. Natl. Acad. Sci. USA contributor: fullname: Baltimore, D. – volume: 295 start-page: 158 year: 1982 end-page: 160 ident: b9 publication-title: Nature contributor: fullname: Melnick, J.L. – volume: 78 start-page: 3403 year: 1981 end-page: 3407 ident: b10 publication-title: Proc. Natl. Acad. Sci. USA contributor: fullname: Shinnick, T.M. – volume: 2 start-page: 177 year: 1984 end-page: 184 ident: b3 publication-title: Vaccine contributor: fullname: Schild, G.C. |
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| Snippet | A synthetic gene coding for the coat protein of tobacco mosaic virus (TMVCP) was expressed in
under the direction of the
UV5 promoter. Modification of the 3'... A synthetic gene coding for the coat protein of tobacco mosaic virus (TMVCP) was expressed in E. coli under the direction of the lac UV5 promoter. Modification... |
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| SubjectTerms | Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry Industrial applications and implications. Economical aspects Production of active biomolecules Vaccins |
| Title | Development of a Genetically-Engineered, Candidate Polio Vaccine Employing the Self-Assembling Properties of the Tobacco Mosaic Virus Coat Protein |
| URI | http://dx.doi.org/10.1038/nbt0786-637 https://www.ncbi.nlm.nih.gov/pubmed/32226216 https://search.proquest.com/docview/2384842268 https://pubmed.ncbi.nlm.nih.gov/PMC7097054 |
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