Inoculum preparation for in-vitro susceptibility testing of filamentous fungi
The percent transmission (T) and the equivalence in cfu/mL of two suspensions of 125 isolates of clinically important filamentous fungi containing 1-5 x 10(5) and 1-5 x 10(4) conidia/mL, adjusted with a haemocytometer, were determined. A wide variation of T values was observed, especially with the f...
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| Published in: | Journal of antimicrobial chemotherapy Vol. 42; no. 3; pp. 385 - 387 |
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| Main Authors: | , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Oxford
Oxford University Press
01-09-1998
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| Abstract | The percent transmission (T) and the equivalence in cfu/mL of two suspensions of 125 isolates of clinically important filamentous fungi containing 1-5 x 10(5) and 1-5 x 10(4) conidia/mL, adjusted with a haemocytometer, were determined. A wide variation of T values was observed, especially with the first inoculum. A good agreement was obtained between the counted and the viable propagules. The haemocytometric method is considered a useful technique for standardizing inocula. |
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| AbstractList | The percent transmission (T) and the equivalence in cfu/mL of two suspensions of 125 isolates of clinically important filamentous fungi containing 1-5 x 10(5) and 1-5 x 10(4) conidia/mL, adjusted with a haemocytometer, were determined. A wide variation of T values was observed, especially with the first inoculum. A good agreement was obtained between the counted and the viable propagules. The haemocytometric method is considered a useful technique for standardizing inocula. The percent transmission (T) and the equivalence in cfu/mL of two suspensions of 125 isolates of clinically important filamentous fungi containing 1-5 x 10 super(5) and 1-5 x 10 super(5) conidia/mL, adjusted with a haemocytometer, were determined. A wide variation of T values was observed, especially with the first inoculum. A good agreement was obtained between the counted and the viable propagules. The haemocytometric method is considered a useful technique for standardizing inocula. |
| Author | FERNANDEZ-BALLART, J PUJOL, I AGUILAR, C GUARRO, J LLOP, C |
| Author_xml | – sequence: 1 givenname: J surname: GUARRO fullname: GUARRO, J organization: Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Spain – sequence: 2 givenname: I surname: PUJOL fullname: PUJOL, I organization: Laboratori de Microbiologia, Hospital Universitari de Sant Joan de Reus, 43201 Reus, Spain – sequence: 3 givenname: C surname: AGUILAR fullname: AGUILAR, C organization: Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Spain – sequence: 4 givenname: C surname: LLOP fullname: LLOP, C organization: Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Spain – sequence: 5 givenname: J surname: FERNANDEZ-BALLART fullname: FERNANDEZ-BALLART, J organization: Unitat de Medicina Preventiva, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Spain |
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| Keywords | Fungi Hemocytometer Filament Sample preparation Methodology Drug susceptibility test Standardization In vitro Thallophyta Quantitative analysis Inoculum |
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| Snippet | The percent transmission (T) and the equivalence in cfu/mL of two suspensions of 125 isolates of clinically important filamentous fungi containing 1-5 x 10(5)... The percent transmission (T) and the equivalence in cfu/mL of two suspensions of 125 isolates of clinically important filamentous fungi containing 1-5 x 10... |
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| SubjectTerms | Acremonium - drug effects Antifungal Agents - pharmacology Biological and medical sciences Colony Count, Microbial Exophiala - drug effects filamentous fungi Fundamental and applied biological sciences. Psychology Fusarium - drug effects Microbial Sensitivity Tests - methods Microbial Sensitivity Tests - standards Microbiology Mycological methods and techniques used in mycology Mycology Paecilomyces - drug effects Spectrophotometry - methods |
| Title | Inoculum preparation for in-vitro susceptibility testing of filamentous fungi |
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