Inoculum preparation for in-vitro susceptibility testing of filamentous fungi

• Published in: Journal of antimicrobial chemotherapy Vol. 42; no. 3; pp. 385 - 387
• Main Authors: GUARRO, J, PUJOL, I, AGUILAR, C, LLOP, C, FERNANDEZ-BALLART, J
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01-09-1998
• Subjects: Acremonium - drug effects; Antifungal Agents - pharmacology; Biological and medical sciences; Colony Count, Microbial; Exophiala - drug effects; filamentous fungi; Fundamental and applied biological sciences. Psychology; Fusarium - drug effects; Microbial Sensitivity Tests - methods; Microbial Sensitivity Tests - standards; Microbiology; Mycological methods and techniques used in mycology; Mycology; Paecilomyces - drug effects; Spectrophotometry - methods; Fungi; Hemocytometer; Filament; Sample preparation; Methodology; Drug susceptibility test; Standardization; In vitro; Thallophyta; Quantitative analysis; Inoculum
• ISSN: 0305-7453; 1460-2091
• DOI: 10.1093/jac/42.3.385

The percent transmission (T) and the equivalence in cfu/mL of two suspensions of 125 isolates of clinically important filamentous fungi containing 1-5 x 10(5) and 1-5 x 10(4) conidia/mL, adjusted with a haemocytometer, were determined. A wide variation of T values was observed, especially with the first inoculum. A good agreement was obtained between the counted and the viable propagules. The haemocytometric method is considered a useful technique for standardizing inocula.