GC–MS fingerprint of Pogostemon cablin in China

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 42; no. 2; pp. 200 - 206
• Main Authors: Hu, L.F., Li, S.P., Cao, H., Liu, J.J., Gao, J.L., Yang, F.Q., Wang, Y.T.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 18-09-2006; Elsevier Science
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; China; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry; GC–MS fingerprint; General pharmacology; Lamiaceae - chemistry; Lamiaceae - growth & development; Medical sciences; Molecular Structure; Oils, Volatile - analysis; Patchouli alcohol; Pharmacology. Drug treatments; Pogostemon cablin; Pogostone; Quality control; Sesquiterpenes - analysis; Asia; China; Pogostemon cablin; Pogostone; GC–MS fingerprint; Patchouli alcohol; Quality control; Labiatae; Essential oil plant; Dicotyledones; Fingerprint method; GC-MS fingerprint; Angiospermae; Alcohol; Spermatophyta
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2005.09.015

Pogostemon cablin, originating in Malaysia and India, is cultivated in southern China including Guangdong and Hainan Province, which was called GuangHuoXiang to differentiate it from the HuoXiang of the north, the species
Agastache rugosa, that it resembles. Essential oil of
P. cablin mainly contributes to the pharmacological activities and the therapeutic properties of the essential oils are directly correlated with their qualitative and quantitative composition. For controlling the quality, standard fingerprint of
P. cablin collected from different regions was developed by using GC–MS. Nine compounds including β-patchoulene, caryophyllene, α-guaiene, seychellene, β-guaiene, δ-guaiene, spathulenol, patchouli alcohol and pogostone were identified among 10 main peaks in
P. cablin. Hierarchical clustering analysis based on characteristics of 10 investigated peaks in GC profiles showed that 18 samples were divided into three main clusters, patchouliol-type, pogostone-type and an interim-type, which was the one between the two chemotypes. The simulative mean chromatogram for the three types
P. cablin was generated using the
Computer Aided Similarity Evaluation System. The fingerprint can help to distinguish the substitute or adulterant, and further assess the differences of
P. cablin grown in various areas of China.