Qualitative and quantitative analyses of nucleosides and nucleobases in Ganoderma spp. by HPLC–DAD-MS

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 44; no. 3; pp. 807 - 811
• Main Authors: Gao, J.L., Leung, Kelvin S.Y., Wang, Y.T., Lai, C.M., Li, S.P., Hu, L.F., Lu, G.H., Jiang, Z.H., Yu, Z.L.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 27-07-2007; Elsevier Science
• Subjects: Adenine - analysis; Adenine - chemistry; Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Fruiting Bodies, Fungal - chemistry; Fundamental and applied biological sciences. Psychology; G. sinense; Ganoderma - chemistry; Ganoderma lucidum; General pharmacology; Guanine - analysis; Guanine - chemistry; HPLC–DAD-MS; Hypoxanthine - analysis; Hypoxanthine - chemistry; Mass Spectrometry - methods; Medical sciences; Molecular Structure; Nucleobase; Nucleoside; Nucleosides - analysis; Nucleosides - chemistry; Pharmacology. Drug treatments; Plant Extracts - chemistry; Quantitative analysis; Reference Standards; Reproducibility of Results; Species Specificity; Technology, Pharmaceutical - methods; Thymine - analysis; Thymine - chemistry; Uracil - analysis; Uracil - chemistry; Asia; China; HPLC–DAD-MS; Nucleoside; Ganoderma lucidum; Nucleobase; G. sinense; Quantitative analysis; HPLC-DAD-MS; Purine derivatives; Chemical analysis; Pharmacognosy; HPLC chromatography; Basidiomycetes; Medicinal plant; Fungi; Folk medicine; Plant origin; Pyrimidine derivatives; Diode array; Qualitative analysis; Thallophyta
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2007.03.012

A high-performance liquid chromatography–diode array detector-mass spectrometry (HPLC–DAD-MS) analytical method was developed for detection of the nucleosides and nucleobases in two species of Lingzhi, the dried sporophore of
Ganoderma lucidum and
G. sinense. The method, combining advantages of both DAD and MS, was successfully used to qualitatively identify for six nucleosides namely, adenosine, cytidine, guanosine, inosine, thymidine, uridine and five nucleobases namely, adenine, guanine, hypoxanthine, thymine and uracil in Lingzhi samples. Quantitative analyses showed that uridine was the most abundant nucleoside in these Lingzhi samples and the contents of nine target analytes were found to be different in pileus and stipes of the fruiting bodies and among the different species of
G. spp. The established method might apply as an alternative approach for the quality assessment of Lingzhi.