First Report of Yersinia ruckeri Biotype 2 in the USA

A polyphasic characterization of atypical isolates of Yersinia ruckeri (causative agent of enteric redmouth disease in trout) obtained from hatchery‐reared brown trout Salmo trutta in South Carolina was performed. The Y. ruckeri isolates were biochemically and genetically distinct from reference cul...

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Bibliographic Details
Published in:	Journal of aquatic animal health Vol. 19; no. 1; pp. 35 - 40
Main Authors:	Arias, Cova R., Olivares‐Fuster, Oscar, Hayden, Karl, Shoemaker, Craig A., Grizzle, John M., Klesius, Phillip H.
Format:	Journal Article
Language:	English
Published:	United States Taylor & Francis Group 01-03-2007
Subjects:	Animals Catfishes - microbiology Disease Outbreaks - veterinary DNA Fingerprinting Fish Diseases - epidemiology Fish Diseases - microbiology Freshwater Gene Amplification Phylogeny Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S - analysis Salmo trutta Species Specificity Trout - microbiology United States - epidemiology Yersinia Infections - epidemiology Yersinia Infections - microbiology Yersinia Infections - veterinary Yersinia ruckeri Yersinia ruckeri - classification Yersinia ruckeri - genetics Yersinia ruckeri - isolation & purification United States USA, South Carolina
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Summary:	A polyphasic characterization of atypical isolates of Yersinia ruckeri (causative agent of enteric redmouth disease in trout) obtained from hatchery‐reared brown trout Salmo trutta in South Carolina was performed. The Y. ruckeri isolates were biochemically and genetically distinct from reference cultures, including the type strain, but were unequivocally ascribed to the species Y. ruckeri, based on API 20E, VITEK, fatty acid methyl ester profiles, and 16S rRNA gene sequencing analysis. These isolates were nonmotile and unable to hydrolyze Tween 20/80 and were therefore classified as Y. ruckeri biotype 2. Genetic fingerprint typing of the isolates via enterobacterial repetitive intergenic consensus (amplified by polymerase chain reaction) and fragment length polymorphism showed biotype 2 as a homogeneous group distinguishable from other Y. ruckeri isolates. This is the first report of Y. ruckeri biotype 2 in the USA.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Case Study-3 ObjectType-Feature-4 ObjectType-Report-2
ISSN:	0899-7659 1548-8667
DOI:	10.1577/H06-011.1