Poly(methylene blue) modified electrode sensor for haemoglobin

• Published in: Analytica chimica acta Vol. 385; no. 1; pp. 119 - 123
• Main Authors: Brett, Christopher M.A, Inzelt, György, Kertesz, Vilmos
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 05-04-1999; Elsevier
• Subjects: Batch injection analysis; Biological and medical sciences; Electrochemical sensor; Haemoglobin; Hematology; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Modified electrodes; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Poly(methylene blue); Modified electrodes; Haemoglobin; Electrochemical sensor; Batch injection analysis; Poly(methylene blue); Performance evaluation; Chemical modification; Analysis method; Hematology; Clinical biology; Measurement sensor; Electrochemistry; Carbon electrode; Hemoglobin; Blood; Quantitative analysis
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/S0003-2670(98)00808-3

Poly(methylene blue) modified electrodes have been prepared by potential cycling from aqueous electrolyte solution containing methylene blue on glassy carbon substrates. They have been used successfully for the determination of haemoglobin via its electrooxidation at fixed potential using the batch injection analysis technique in which whole blood samples diluted with phosphate or acetate buffer are injected directly over the modified electrode immersed in inert electrolyte. Electrode response is fast, stable, and reproducible. For blood from healthy donors, a good linear correlation was found between the results from the electrochemical injection method and the conventional clinical spectrophotometric measurement after cyanidation. The blood from potentially ill donors showed deviations from the linear correlation suggesting that this may be a good method for detecting haematological problems.