Sequence analysis of KmXYL1 genes and verification of thermotolerant enzymatic activities of xylose reductase from four Kluyveromyces marxianus strains

Kluyveromyces marxianus has the capability of producing xylitol from xylose because of the endogenous xylose reductase ( KmXYL1 ) gene. In this study, we cloned KmXYL1 genes and compared amino acid sequences of xylose reductase (XR) from four K. marxianus strains (KCTC 7001, KCTC 7155, KCTC 17212, a...

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Bibliographic Details
Published in:	Biotechnology and bioprocess engineering Vol. 21; no. 5; pp. 581 - 586
Main Authors:	Park, Jae-Bum, Kim, Jin-Seong, Jang, Seung-Won, Kweon, Deok-Ho, Hong, Eock Kee, Shin, Won Cheol, Ha, Suk-Jin
Format:	Journal Article
Language:	English
Published:	Seoul The Korean Society for Biotechnology and Bioengineering 01-09-2016 Springer Nature B.V 한국생물공학회
Subjects:	Amino acids Bioengineering Biomass Biotechnology Cell growth Chemistry Chemistry and Materials Science Cloning Enzymatic activity Enzymes Ethanol Experiments Fermentation Gene expression Genes High temperature Industrial and Production Engineering Kluyveromyces marxianus Research Paper Studies Yeast 생물공학 thermotolerant xylose reductase
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Summary:	Kluyveromyces marxianus has the capability of producing xylitol from xylose because of the endogenous xylose reductase ( KmXYL1 ) gene. In this study, we cloned KmXYL1 genes and compared amino acid sequences of xylose reductase (XR) from four K. marxianus strains (KCTC 7001, KCTC 7155, KCTC 17212, and KCTC 17555). Four K. marxianus strains showed high homologies (99%) of amino acid sequences with those from other reported K. marxianus strains and around 60% homologies with that from Scheffersomyces stipitis . For XR enzymatic activities, four K. marxianus strains exhibited thermostable XR activities up to 45°C and K. marxianus KCTC 7001 showed the highest XR activity. When reaction temperatures were increased from 30 to 45°C, NADH-dependent XR activity from K. marxianus KCTC 7001 was highly increased (46%). When xylitol fermentations were performed at 30 or 45°C, four K. marxianus strains showed very poor xylitol production capabilities regardless fermentation temperatures. Xylitol productions from four K. marxianus strains might be limited because of low xylose uptake rate or cell growth although they have high thermostable XR activities.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 G704-000785.2016.21.5.005
ISSN:	1226-8372 1976-3816
DOI:	10.1007/s12257-016-0363-6