Functional protein microarrays: ripe for discovery

The manufacture and use of protein microarrays with correctly folded and functional content presents significant challenges. Despite this, the feasibility and utility of such undertakings are now clear, and exciting progress has recently been demonstrated in the areas of content generation, printing...

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Bibliographic Details
Published in:	Current opinion in chemical biology Vol. 8; no. 1; pp. 8 - 13
Main Author:	Predki, Paul F
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-02-2004
Subjects:	Animals Humans Phosphorylation Protein Array Analysis - methods Protein Binding Proteome - genetics Proteome - immunology Proteome - metabolism Proteomics
Online Access:	Get full text
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Summary:	The manufacture and use of protein microarrays with correctly folded and functional content presents significant challenges. Despite this, the feasibility and utility of such undertakings are now clear, and exciting progress has recently been demonstrated in the areas of content generation, printing strategies and protein immobilization. More importantly, we are now beginning to enjoy the fruits of these efforts as functional protein microarrays are being increasingly employed for biological discovery purposes. Recent examples of this include the characterization of autoantibody responses, antibody specificity profiling, protein–protein domain interaction profiling and a comprehensive characterization of coiled-coil interactions. The best, however, is yet to come.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 ObjectType-Review-3 content type line 23 ObjectType-Feature-3 ObjectType-Review-1
ISSN:	1367-5931 1879-0402
DOI:	10.1016/j.cbpa.2003.12.005