Evaluation of the optimum dietary arachidonic acid level and its essentiality based on growth and non-specific immune responses in Japanese eel, Anguilla japonica

• Published in: Aquaculture Vol. 452; pp. 209 - 216
• Main Authors: Shahkar, Erfan, Yun, Hyeonho, Lee, Seunghan, Kim, Dae-Jung, Kim, Shin-Kwon, Lee, Bae Ik, Bai, Sungchul C.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01-02-2016; Elsevier Sequoia S.A
• Subjects: Anguilla japonica; Aquaculture; Arachidonic acid; Fatty acids; Fish; Growth; Innate immunity; Japanese eel; Marine; Regression analysis; INW, Japan; Japanese eel; Innate immunity; Anguilla japonica; Arachidonic acid; Growth
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/j.aquaculture.2015.10.034

A feeding trial was conducted to evaluate the optimum dietary level and the essentiality of arachidonic acid (ARA, 20:4n-6) based on growth and non-specific immune responses in Japanese eel, Anguilla japonica. A basal ARA free commercial diet was used as a control, and four other diets were prepared by supplementing 0.3, 0.6, 0.9 or 1.5% ARA. The analyzed ARA concentrations of the diets were 0.00, 0.33, 0.71, 1.06 and 1.65% diet (ARA0, ARA0.33, ARA0.71, ARA1.06 and ARA1.65), respectively. Triplicate groups of fish (initial body weight, 157±2.5g) were fed one of 5 experimental diets at a ratio of 4% body weight for 12weeks. At the end of the feeding trial, final weight, weight gain, specific growth rate and feed efficiency of fish fed the ARA0.71 diet were significantly higher than those of fish fed the ARA0, ARA0.33 and ARA1.65 diets (P<0.05). The polynomial regression analysis of weight gain indicates that the optimum dietary ARA level could be 0.92% diet. Superoxide dismutase activity of fish fed the ARA1.65 diet was significantly higher than those of fish fed the ARA0 and ARA0.33 diets. However, there were no significant differences in superoxide dismutase activity among fish fed diets ARA0.71, ARA1.06 and ARA1.65. Fish fed the ARA1.06 diet showed significantly higher lysozyme activity than those of fish fed the ARA0 and ARA0.33 diets. However, there were no significant differences in lysozyme activity among fish fed the ARA0.71, ARA1.06 and ARA1.65 diets. Therefore, the optimum dietary ARA level could be greater than 0.71% but less than 0.92%. Also, these results may indicate that dietary ARA could be one of the essential fatty acids in Japanese eel.
Statement of relevance
The findings showed the essentiality of an appropriate arachidonic acid supplementation level for optimum growth of Japanese eel.
•Optimum dietary arachidonic acid level and the essentiality of fatty acid in the diet of Japanese eel•Growth performance was significantly influenced by dietary arachidonic acid levels.•Dietary arachidonic acid supplementation augmented the non-specific immune response of fish.•Increments in the levels of dietary arachidonic acid increased lipid peroxidation.•Tissue arachidonic acid concentration reflected the dietary arachidonic acid levels.