Isolation and characterization of microsatellite markers for Cedrela odorata L. (Meliaceae), a high value neotropical tree

• Published in: Conservation genetics Vol. 9; no. 2; pp. 457 - 459
• Main Authors: Hernández, G, Buonamici, A, Walker, K, Vendramin, G. G, Navarro, C, Cavers, S
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01-04-2008; Springer Netherlands; Springer Nature B.V
• Subjects: Animal Genetics and Genomics; Biodiversity; Biomedical and Life Sciences; Cedar; Cedrela odorata; Conservation biology; Conservation Biology/Ecology; Ecology; Evolutionary Biology; Genetic markers; Life Sciences; Meliaceae; microsatellite repeats; Plant Genetics and Genomics; Technical Note; Trees; Cedar; Meliaceae; Microsatellites
• ISSN: 1566-0621; 1572-9737
• DOI: 10.1007/s10592-007-9334-y

We describe 9 primers for amplification of microsatellite loci for the Neotropical tree Cedrela odorata L. (Meliaceae). Loci were isolated from an enriched library derived from a single DNA sample from a tree in Costa Rica. Levels of polymorphism were determined using samples from a large progeny trial. Across loci, the number of alleles ranged from 14 to 30. Observed heterozygosity levels ranged from 0.61 to 0.88. No linkage disequilibria were detected although some departures from Hardy-Weinberg equilibrium (HWE) were found, probably due to a Wahlund effect.