Adaptation and validation of antibody-ELISA using dried blood spots on filter paper for epidemiological surveys of tsetse-transmitted trypanosomosis in cattle

The indirect enzyme-linked immunosorbent assay (ELISA) for the detection of anti-trypanosomal antibodies in bovine serum was adapted for use with dried blood spots on filter paper. Absorbance (450 nm) results for samples were expressed as percent positivity, i.e. percentage of the median absorbance...

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Bibliographic Details
Published in:	Preventive veterinary medicine Vol. 37; no. 1; pp. 91 - 99
Main Authors:	Hopkins, Julian S, Chitambo, Harrison, Machila, Noreen, Luckins, Anthony G, Rae, Phillip F, van den Bossche, Peter, Eisler, Mark C
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-12-1998
Subjects:	Animals ANTIBODIES Antibodies, Protozoan - blood Antibody ANTICORPS ANTICUERPOS BOVIN CATTLE Cattle parasitological diseases DIAGNOSIS DIAGNOSTIC DIAGNOSTICO DISEASE SURVEYS ELISA ENCUESTAS SANITARIAS ENQUETE PATHOLOGIQUE Enzyme-Linked Immunosorbent Assay - methods GANADO BOVINO Prevalence Reproducibility of Results Sensitivity Seroepidemiologic Studies Specificity TEST ELISA TRIPANOSOMIASIS TRYPANOSOMA BRUCEI TRYPANOSOMA CONGOLENSE TRYPANOSOMA VIVAX TRYPANOSOMIASIS Trypanosomiasis, African - veterinary Trypanosomiasis, Bovine - diagnosis Trypanosomiasis, Bovine - epidemiology Trypanosomiasis, Bovine - immunology TRYPANOSOMOSE Trypanosomosis ZAMBIA Zambia - epidemiology ZAMBIE Zambia Cattle parasitological diseases Sensitivity Specificity Antibody Diagnosis ELISA Trypanosomosis
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