De-emulsification of oil-in-water emulsions by Bacillus subtilis

Bacillus subtilis grown on glucose produced a de-emulsifier that broke down model oil-in-water emulsions. The deemulsification increased with contact time and the recovery of oil was 98% with 0.6 mg de-emulsifier/ml emulsion. GLC and i.r. analysis of the molecule extracted from cell-free broth demon...

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Bibliographic Details
Published in:	World journal of microbiology & biotechnology Vol. 10; no. 4; pp. 452 - 456
Main Authors:	JANIYANI, K. L, PUROHIT, H. J, SHANKER, R, KHANNA, P
Format:	Journal Article
Language:	English
Published:	Dordrecht Springer 01-07-1994
Subjects:	Bacillus subtilis Bacteriology Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Metabolism. Enzymes Microbiology Culture medium Microorganism growth Bacillus subtilis Demulsifying agent Oil water emulsion Metabolite 2-Butanone(3-hydroxy) Glucose Bacillaceae Oil base mud Infrared spectrometry Bacillales Production Microorganism culture Bacteria Total organic carbon Gas liquid chromatography Quantitative analysis
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Summary:	Bacillus subtilis grown on glucose produced a de-emulsifier that broke down model oil-in-water emulsions. The deemulsification increased with contact time and the recovery of oil was 98% with 0.6 mg de-emulsifier/ml emulsion. GLC and i.r. analysis of the molecule extracted from cell-free broth demonstrated that the de-emulsifier was acetoin. It may be better to use acetoin, a metabolite of glucose metabolism, to recover oil from oily sludges instead of microbial cells, because of its stability and de-emulsification activity.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0959-3993 1573-0972
DOI:	10.1007/BF00144471