Carotenoid biosynthesis in wild type and mutant strains of Mucor circinelloides

• Published in: Biochimica et biophysica acta Vol. 1289; no. 2; pp. 203 - 208
• Main Authors: Fraser, Paul D, Ruiz-Hidalgo, Maria Jose, Lopez-Matas, Maria Angeles, Alvarez, Maria Isabel, Eslava, Arturo P, Bramley, Peter M
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15-03-1996
• Subjects: Carotenoid; Carotenoids - biosynthesis; Isoprenoid; Mevalonic Acid - metabolism; Mucor - metabolism; Mucor circinelloides; Mutant; Mutation; Polyisoprenyl Phosphates - metabolism; FDP; DPA; IDP; Mutant; Mucor circinelloides; MVA; Isoprenoid; AMO 1618; Carotenoid; GGDP
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/0304-4165(95)00169-7

Carotenoid biosynthesis in wild type
Mucor circinelloides has been investigated and the biochemical characterisation of the MS1 and MS9 mutant strains, impaired in carotenoid formation, carried out. In liquid cultures, all strains produced carotenoids (mainly β-carotene. but also ζ-carotene, lycopene and γ-carotene) at the onset of stationary phase of growth. Carotenogenesis was light dependent. In liquid cultures carotenoid formation in wild type was affected by diphenylamine, which prevented desaturation, nicotine, resulting in reduced carotenoid levels, but CPTA caused an increase in the total carotenoid content but a reduced β-carotene level, with the accumulation of lycopene and γ-carotene. The mutant strains MS1 and MS9 contained only 5.0 and 11.5% of wild type carotenoid levels, respectively. Cell extracts of light-grown mycelia, incubated with 3(
R)-[2-
14C] mevalonic acid, produced β-carotene, but incorporations into carotenoids were substantially reduced in the cell extracts of MS1 and MS9. Analysis of prenyl diphosphate intermediates indicated that. compared to wild type, geranylgeranyl diphosphate accumulated in MS1. MS9 extracts produced a larger amount of prenyl phosphates and a more even distribution of radioactivity from mevalopic acid into farnesyl and geranylgeranyl diphosphates. Squalene and long chain prenyl phosphates were formed by the cell extracts of all strains. It is proposed that the MS1 strain possesses a mutation in a gene responsible for phytoene formation, whilst a regulatory mutation, affecting prenyl transferase activities, has occurred in MS9.