Small-angle X-ray scattering study of the influence of solvent replacement (from H2O to D2O) on the initial crystallization stage of tetragonal lysozyme

• Published in: Crystallography reports Vol. 62; no. 6; pp. 837 - 842
• Main Authors: Boikova, A. S., D’yakova, Yu. A., Il’ina, K. B., Konarev, P. V., Kryukova, A. E., Marchenkova, M. A., Blagov, A. E., Pisarevskii, Yu. V., Koval’chuk, M. V.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-11-2017; Springer Nature B.V
• Subjects: COMPARATIVE EVALUATIONS; Concentration (composition); CONCENTRATION RATIO; CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; Crystallography and Scattering Methods; CRYSTALS; Diffraction and Scattering of Ionizing Radiations; HEAVY WATER; INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; Light water; LYSOZYME; MONOMERS; Physics; Physics and Astronomy; SMALL ANGLE SCATTERING; Small angle X ray scattering; Solvents; SYNCHROTRON RADIATION; X-RAY DIFFRACTION
• ISSN: 1063-7745; 1562-689X
• DOI: 10.1134/S1063774517060074

The composition of lysozyme solutions in D
2
O under conditions favorable for the formation of tetragonal crystals has been investigated at different protein concentrations by small-angle X-ray scattering using the synchrotron radiation. In addition to lysozyme monomers, dimeric and octameric species are found in the crystallization solutions; the octamer content increases with an increase in the protein concentration. A comparison of the data with those obtained under similar conditions but with H
2
O used as a solvent has shown that the replacement of light water with heavy one leads to increase of octamer volume fraction in solution.