Composition of the inner mitochondrial membrane of porcine corpus luteum

• Published in: Biochimica et biophysica acta Vol. 1022; no. 3; p. 401
• Main Authors: Holland, J W, Stevenson, P M
• Format: Journal Article
• Language: English
• Published: Netherlands 01-03-1990
• Subjects: Animals; Cardiolipins - analysis; Cholesterol - analysis; Cholesterol Side-Chain Cleavage Enzyme - analysis; Corpus Luteum - ultrastructure; Cytochromes - analysis; Fatty Acids - analysis; Female; Intracellular Membranes - analysis; Mitochondria - ultrastructure; Phospholipids - analysis; Swine; Ubiquinone - analysis
• ISSN: 0006-3002
• DOI: 10.1016/0005-2736(90)90291-U

An inner mitochondrial membrane fraction was prepared from porcine corpus luteum. The concentrations of the respiratory cytochromes, cytochrome P-450scc, cholesterol, ubiquinone, cardiolipin and the total phospholipids were measured. The fatty acid compositions of cardiolipin and the total phospholipid fraction were determined. Comparative data from porcine heart and liver were obtained using the same methods. Differences in both the concentration and the fatty acid composition of the phospholipids were observed between the tissues. It appeared that the phospholipid bilayer was expanded relative to haem a in luteal mitochondria. It is proposed that in the ovary this expansion may be necessary to accommodate cytochrome P-450scc and its substrate, cholesterol.